Zhang Hui, Yan Lei, Bai Yun, Li Chunyan, Guo Qiufen, Wang Chong, Zhao Xingbo, Li Mingjiang
Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
Department of General Surgery, Shandong Rongjun General Hospital, 23 Jiefang Road, Jinan, Shandong 250013, People's Republic of China.
Gynecol Oncol. 2015 Mar;136(3):549-53. doi: 10.1016/j.ygyno.2014.11.008. Epub 2014 Nov 14.
We previously found that Dual-specificity phosphatase 6 (Dusp6) over-expression enhanced the growth-promoting effect of estrogen in endometrial adenocarcinoma cells. The aim of this study was to explore the correlation of Dusp6 expression with progestin sensitivity in atypical endometrial hyperplasia (AEH) and earlier endometrial carcinomas (EC).
Using immunohistochemistry study, we analyzed the expression of Dusp6 protein in AEH.
We found that progestin treatment was effective in 89% of AEH and 50% of EC. Before treatment, Dusp6 expression was significantly higher in progestin-sensitive AEH groups compared with progestin-resistant groups. After treatment, Dusp6 expression was significantly upregulated in progestin-sensitive groups, but not in progestin-resistant groups. Moreover, a high-dose of Dusp6 transfection significantly enhanced progestin-induced growth-inhibition in Ishikawa cells.
Dusp6 could be a predicting marker for deciding the effectiveness of progestin therapy in AEH.
我们之前发现双特异性磷酸酶6（Dusp6）的过表达增强了雌激素对子宫内膜腺癌细胞的促生长作用。本研究旨在探讨Dusp6表达与非典型子宫内膜增生（AEH）及早期子宫内膜癌（EC）中孕激素敏感性之间的相关性。
采用免疫组织化学研究，分析Dusp6蛋白在AEH中的表达。
我们发现孕激素治疗对89%的AEH和50%的EC有效。治疗前，孕激素敏感的AEH组中Dusp6表达显著高于孕激素抵抗组。治疗后，孕激素敏感组中Dusp6表达显著上调，而孕激素抵抗组中则未上调。此外，高剂量的Dusp6转染显著增强了孕激素对Ishikawa细胞的生长抑制作用。
Dusp6可能是决定孕激素治疗AEH有效性的一个预测标志物。
