秀丽隐杆线虫中X染色体的差异空间和结构组织是剂量补偿的基础。
Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans.
作者信息
Sharma Rahul, Jost Daniel, Kind Jop, Gómez-Saldivar Georgina, van Steensel Bas, Askjaer Peter, Vaillant Cédric, Meister Peter
机构信息
Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland;
Laboratoire de Physique, Ecole Normale Supérieure de Lyon, UMR 5672, Centre National de la Recherche Scientifique (CNRS), 69007 Lyon, France;
出版信息
Genes Dev. 2014 Dec 1;28(23):2591-6. doi: 10.1101/gad.248864.114.
The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model of chromosome-wide gene regulation. In Caenorhabditis elegans, DC is achieved by twofold down-regulation of gene expression from both Xs in hermaphrodites. We show that in males, the single X chromosome interacts with nuclear pore proteins, while in hermaphrodites, the DC complex (DCC) impairs this interaction and alters X localization. Our results put forward a structural model of DC in which X-specific sequences locate the X chromosome in transcriptionally active domains in males, while the DCC prevents this in hermaphrodites.
X连锁基因表达对X染色体拷贝数的调整（剂量补偿[DC]）已作为全染色体范围基因调控的模型被广泛研究。在秀丽隐杆线虫中，雌雄同体的两条X染色体上的基因表达均下调两倍来实现剂量补偿。我们发现，在雄性个体中，单条X染色体与核孔蛋白相互作用，而在雌雄同体中，剂量补偿复合体（DCC）会破坏这种相互作用并改变X染色体的定位。我们的研究结果提出了一种剂量补偿的结构模型，即X特异性序列将雄性个体中的X染色体定位在转录活性区域，而在雌雄同体中DCC会阻止这种情况发生。
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