Kotliar B I, Miasnikov A A, Korshunov V A
Nauchnye Doki Vyss Shkoly Biol Nauki. 1989(3):56-63.
The difficulties in investigation of the single neuron integrative properties in mammalian brain are connected with the absence of a mean of determination of neuronal set contribution in their activity. In given paper an experimental approach to the decision of this task is proposed. It consists in neuron activity investigation in the model learning training situation under condition of partial functional isolation from nervous cells by means of decrease in extracellular calcium concentration level using ethylene glycol tetraacetate. As indicator of such isolation was a partial or total reduction of responses in investigated neurons to electric microstimulation of neighbouring (200-240 mkm) parts of neocortex. The results of analysis of neuron responses in sensomotor cortex in the rat's brain in the process of acetylcholine repetitive local application give possibility to propose that some neurons don't exhibit plasticity according to the indicator in the dynamics of impulse discharge rate in responses to transmitter.
在哺乳动物大脑中研究单个神经元整合特性的困难与缺乏确定神经元群体对其活动贡献的方法有关。在本文中，提出了一种解决该任务的实验方法。它包括在模型学习训练情境中，通过使用乙二醇四乙酸降低细胞外钙浓度水平，在与神经细胞部分功能隔离的条件下研究神经元活动。这种隔离的指标是被研究神经元对新皮层相邻（200 - 240微米）部分的电微刺激反应的部分或完全减少。对大鼠大脑感觉运动皮层中神经元在乙酰胆碱重复局部应用过程中的反应分析结果表明，根据对递质反应中冲动发放率动态的指标，一些神经元不表现出可塑性。
