建立一株从人骨肉瘤中产生骨形态发生蛋白的细胞系。

Establishment of a cell line producing bone morphogenetic protein from a human osteosarcoma.

作者信息

Takaoka K, Yoshikawa H, Masuhara K, Sugamoto K, Tsuda T, Aoki Y, Ono K, Sakamoto Y

机构信息

Department of Orthopaedic Surgery, Osaka University Medical School, Japan.

出版信息

Clin Orthop Relat Res. 1989 Jul(244):258-64.

PMID:2545399

Abstract

A human osteosarcoma cell line was established from a biopsy specimen from a 13-year-old girl. The osteosarcoma tissue was maintained in athymic nude mice (Balb C nu/nu) by serial transplantation for three years. The tumor was excised from a host mouse and digested with collagenase. The isolated cells were cultured by 98 passages in 14 months, and clones of osteosarcoma cells were obtained by limiting dilution. A clone named human osteosarcoma cell 6 (H-OS-6) that showed the osteoblastic phenotypes of productions of bone morphogenetic protein (BMP) and alkaline phosphatase and a response to human parathyroid hormone (h-PTH 1-34) was selected. The morphology of its chromosomes indicated its human origin. This human osteosarcoma cell line is unique in producing BMP under in vitro conditions.

摘要

一株人骨肉瘤细胞系源自一名13岁女孩的活检标本。骨肉瘤组织通过连续移植在无胸腺裸鼠（Balb C nu/nu）体内维持了三年。从宿主小鼠身上切除肿瘤并用胶原酶消化。分离出的细胞在14个月内传代培养了98次，并通过有限稀释法获得了骨肉瘤细胞克隆。筛选出一个名为人类骨肉瘤细胞6（H-OS-6）的克隆，该克隆表现出成骨细胞表型，可产生骨形态发生蛋白（BMP）和碱性磷酸酶，并对人甲状旁腺激素（h-PTH 1-34）有反应。其染色体形态表明其来源于人类。该人骨肉瘤细胞系在体外条件下产生BMP具有独特性。