Shoieb A M, Hahn K A, Barnhill M A
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071, USA.
In Vivo. 1998 Sep-Oct;12(5):463-72.
In this report we describe the establishment, characterization, and research utility of a cell line derived from a dog having a spontaneously occurring osteosarcoma.
Tumor samples were collected from a dog with a naturally occurring osteosarcoma and processed for light microscopy, electron microscopy, immunocytochemistry, immunohistochemistry, karyology, and cell culture. Established cells from passage 31 (COS31; canine Qsteosarcoma cells from passage 31) were inoculated subcutaneously between the scapula and in the right abdominal side of athymic nude mice and evaluated similarly.
COS31 cells derived in cell culture and in nude mice had morphological and biochemical properties comparable in all respects to the original canine tumor specimen.
The ability of COS31 cells to produce tumors in nude mice (i.e. a small animal model) typical of canine osteosarcoma (i.e. a large animal model) with a similar pathological and biological behavior (e.g. alkaline phosphatase and osteocalcin positive immunostaining, osteoid production, rapid growth, and wide spread metastases) demonstrates the potential utility of COS31 cells as a in vitro and in vivo model system in the development of new strategies in the treatment of human osteosarcoma.
在本报告中，我们描述了一种源自患有自发性骨肉瘤的犬类的细胞系的建立、特性及研究用途。
从一只患有自然发生的骨肉瘤的犬类收集肿瘤样本，并进行光学显微镜检查、电子显微镜检查、免疫细胞化学、免疫组织化学、核型分析及细胞培养。将传代31代的已建立细胞（COS31；传代31代的犬骨肉瘤细胞）接种到无胸腺裸鼠的肩胛骨间及右侧腹部皮下，并进行类似评估。
在细胞培养物及裸鼠中产生的COS31细胞在所有方面均具有与原始犬类肿瘤标本相当的形态学和生化特性。
COS31细胞在裸鼠（即小动物模型）中产生典型犬骨肉瘤（即大动物模型）肿瘤的能力，且具有相似的病理和生物学行为（如碱性磷酸酶和骨钙素阳性免疫染色、类骨质生成、快速生长及广泛转移），这表明COS31细胞作为体外和体内模型系统在开发人类骨肉瘤治疗新策略方面具有潜在用途。
