基于包裹在酶促合成的聚（1,10-菲咯啉-5,6-二酮）内的葡萄糖氧化酶的电化学生物传感器。
Electrochemical biosensor based on glucose oxidase encapsulated within enzymatically synthesized poly(1,10-phenanthroline-5,6-dione).
作者信息
Ciftci Hakan, Oztekin Yasemin, Tamer Ugur, Ramanaviciene Almira, Ramanavicius Arunas
机构信息
Kirikkale University, Kirikkale Vocational High School, Department of Chemistry and Chemical Processing Technologies, Kirikkale, Turkey; Vilnius University, Faculty of Chemistry, NanoTechnas - Centre of Nanotechnology and Materials Science and Department of Physical Chemistry, Vilnius, Lithuania; State Research Institute, Center for Physical Sciences and Technology, Laboratory of Bio-nanotechnology, Vilnius Lithuania.
Vilnius University, Faculty of Chemistry, NanoTechnas - Centre of Nanotechnology and Materials Science and Department of Physical Chemistry, Vilnius, Lithuania; State Research Institute, Center for Physical Sciences and Technology, Laboratory of Bio-nanotechnology, Vilnius Lithuania; Selcuk University, Faculty of Science, Department of Chemistry, Konya, Turkey.
出版信息
Colloids Surf B Biointerfaces. 2014 Nov 1;123:685-91. doi: 10.1016/j.colsurfb.2014.10.032. Epub 2014 Oct 24.
This study is focused on the investigation of electrocatalytic effect of glucose oxidase (GOx) immobilized on the graphite rod (GR) electrode. The enzyme modified electrode was prepared by encapsulation of immobilized GOx within enzymatically formed poly(1,10-phenanthroline-5,6-dione) (pPD) film. The electrochemical responses of such enzymatic electrode (pPD/GOx/GR) vs. different glucose concentrations were examined chronoamperometrically in acetate-phosphate buffer solution (A-PBS), pH 6.0, under aerobic or anaerobic conditions. Amperometric signals of the pPD/GOx/GR electrode exhibited well-defined hyperbolic dependence upon glucose concentration. Amperometric signals at 100mM of glucose were 41.17 and 32.27 μA under aerobic and anaerobic conditions, respectively. Amperometric signals of the pPD/GOx/GR electrode decreased by 6% within seven days. The pPD/GOx/GR electrode showed excellent selectivity in the presence of dopamine and uric acid. Furthermore it had a good reproducibility and repeatability with standard deviation of 9.4% and 8.0%, respectively.
本研究聚焦于对固定在石墨棒（GR）电极上的葡萄糖氧化酶（GOx）的电催化效应进行研究。酶修饰电极是通过将固定化的GOx包封在酶促形成的聚（1,10 - 菲咯啉 - 5,6 - 二酮）（pPD）膜中制备而成。在有氧或无氧条件下，于pH 6.0的醋酸盐 - 磷酸盐缓冲溶液（A - PBS）中，采用计时电流法检测该酶电极（pPD/GOx/GR）对不同葡萄糖浓度的电化学响应。pPD/GOx/GR电极的安培信号对葡萄糖浓度呈现出明确的双曲线依赖性。在有氧和无氧条件下，葡萄糖浓度为100mM时的安培信号分别为41.17μA和32.27μA。pPD/GOx/GR电极的安培信号在七天内下降了6%。pPD/GOx/GR电极在多巴胺和尿酸存在的情况下表现出优异的选择性。此外，它具有良好的重现性和重复性，标准偏差分别为9.4%和8.0%。
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