Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand; Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Thailand.
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
Talanta. 2017 May 1;166:420-427. doi: 10.1016/j.talanta.2015.11.072. Epub 2015 Nov 30.
We report a novel amperometric glucose biosensor based on glucose oxidase (GOx) immobilized on a carbon nanotube (CNTs)-poly(diallyldimethyl-ammonium chloride) (PDDA)-platinum nanoparticle (PtNPs) modified carbon-paste electrode (CNTs-PDDA-PtNPs/CPE). The CNTs-PDDA-PtNPs composite materials were characterized by TEM and electrochemical techniques. Cyclic voltammetric results reveal direct electron transfer of the immobilized GOx, indicated by two quasi-reversible redox peaks at a potential of 0.37V (vs. Ag/AgCl) in phosphate buffered solution (PBS) (0.10M, pH 7). The biosensor provides good glucose oxidation activity and retention of GOx electrocatalytic activity due to CNTs-PDDA-PtNPs enhancement of the redox response. The carbon paste electrode was installed as working electrode in a flow through electrochemical cell of a flow injection (FI) system. Glucose was quantified using amperometric measurements at 0.5V vs. Ag/AgCl and PBS carrier (0.10M, pH 7.0) at a flow rate of 1.0mLmin. The linear working ranges for glucose measurements were 0.1-3mM (r=0.995) and 5-100mM (r=0.997), with corresponding sensitivities of 0.127 and 0.060 (μAs) mM, respectively. The system provides good precision of 2.8% R.S.D with a calculated detection limit (3S/N) of 15μM. The proposed method was successfully applied to determination of glucose in food and pharmaceutical samples with throughput of 200 samplesh.
我们报道了一种基于葡萄糖氧化酶（GOx）固定在碳纳米管（CNTs）-聚二烯丙基二甲基氯化铵（PDDA）-铂纳米粒子（PtNPs）修饰的碳糊电极（CNTs-PDDA-PtNPs/CPE）上的新型安培葡萄糖生物传感器。通过 TEM 和电化学技术对 CNTs-PDDA-PtNPs 复合材料进行了表征。循环伏安结果表明，固定化 GOx 发生了直接电子转移，在磷酸盐缓冲溶液（PBS）（0.10M，pH7）中，在 0.37V（相对于 Ag/AgCl）处出现了两个准可逆氧化还原峰。由于 CNTs-PDDA-PtNPs 增强了氧化还原响应，该生物传感器提供了良好的葡萄糖氧化活性和 GOx 电催化活性的保留。将碳糊电极安装在流动注射（FI）系统的流通式电化学池中作为工作电极。在 0.5V（相对于 Ag/AgCl）和 PBS 载体（0.10M，pH7.0）下，使用安培测量法对葡萄糖进行定量，流速为 1.0mLmin。葡萄糖测量的线性工作范围为 0.1-3mM（r=0.995）和 5-100mM（r=0.997），相应的灵敏度分别为 0.127 和 0.060（μAs）mM。该系统提供了良好的精度，2.8%R.S.D，计算出的检测限（3S/N）为 15μM。该方法成功应用于食品和药物样品中葡萄糖的测定，通量为 200 样/h。
