Salamifar S Ehsan, Lee Stephen, Lai Rebecca Y
Department of Chemistry University of Nebraska-Lincoln, 651 Hamilton Hall, Lincoln, NE 68588-0304, USA.
Department of Chemistry University of Nebraska-Lincoln, 651 Hamilton Hall, Lincoln, NE 68588-0304, USA.
Colloids Surf B Biointerfaces. 2014 Nov 1;123:866-9. doi: 10.1016/j.colsurfb.2014.10.033. Epub 2014 Oct 28.
We report the design and fabrication of hydrogen peroxide (H2O2) sensors using heme proteins immobilized on macroelectrodes and ultramicroelectrodes (UMEs). In this sensor design, the heme centers are directly "wired" to the electrode via the use of an imidazole-terminated self-assembled monolayer. We have systematically evaluated the effect of electrode type and size on sensor performance. The limit of detection for H2O2 determined using a 10-μm gold UME is significantly lower than that obtained using a stationary macroelectrode. Our results also highlight the advantages of using UMEs for enzyme kinetics analysis; the Km determined using a 10-μm UME is similar to that obtained from a rotating disk electrode.
我们报告了使用固定在宏观电极和超微电极（UME）上的血红素蛋白设计和制造过氧化氢（H2O2）传感器的情况。在这种传感器设计中，血红素中心通过使用咪唑端基自组装单分子层直接与电极“连接”。我们系统地评估了电极类型和尺寸对传感器性能的影响。使用10μm金超微电极测定的H2O2检测限明显低于使用固定宏观电极获得的检测限。我们的结果还突出了使用超微电极进行酶动力学分析的优势；使用10μm超微电极测定的Km与从旋转圆盘电极获得的Km相似。
