Meddens Marjolein B M, van den Dries Koen, Cambi Alessandra
Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein Zuid 26-28, 6525 GA Nijmegen, The Netherlands.
Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein Zuid 26-28, 6525 GA Nijmegen, The Netherlands.
Eur J Cell Biol. 2014 Oct;93(10-12):380-7. doi: 10.1016/j.ejcb.2014.09.002. Epub 2014 Oct 13.
Podosomes are micrometer-sized, circular adhesions formed by cells such as osteoclasts, macrophages, dendritic cells, and endothelial cells. Because of their small size and the lack of methods to visualize individual proteins and protein complexes, podosomes have long been considered a simple two-module structure with a protrusive actin core and a surrounding adhesive ring composed of integrins and cytoskeletal adaptor proteins such as vinculin and talin. In the past decade, the applications of fluorescence based techniques that circumvent the diffraction limit of conventional light microscopy took a major leap forward. Podosomes have been imaged by a variety of these super-resolution methods, and in this concise review we discuss how these super-resolution data have increased our understanding of the podosome ultra-structure and function.
足体是由破骨细胞、巨噬细胞、树突状细胞和内皮细胞等细胞形成的微米级圆形黏附结构。由于其尺寸小，且缺乏可视化单个蛋白质和蛋白质复合物的方法，足体长期以来一直被认为是一种简单的双模块结构，具有突出的肌动蛋白核心和由整合素以及诸如纽蛋白和踝蛋白等细胞骨架衔接蛋白组成的周围黏附环。在过去十年中，基于荧光技术的应用突破了传统光学显微镜的衍射极限，取得了重大进展。通过多种这些超分辨率方法对足体进行了成像，在这篇简要综述中，我们讨论了这些超分辨率数据如何增进了我们对足体超微结构和功能的理解。
