Severin J, Langel P, Höfer M
Botanisches Institut der Universität, Bonn, FRG.
J Bioenerg Biomembr. 1989 Jun;21(3):321-34. doi: 10.1007/BF00762724.
H+/sugar symport in the obligatory aerobic yeast Rhodotorula glutinis was analyzed under conditions where the plasma membrane was selectively depolarized by the lipophilic cation tetraphenylphosphonium (TPP+). Control experiments showed that this treatment did not impair the transmembrane delta pH, the cell energy charge, and the function of plasma membrane H+-ATPase. The kinetic data were fitted to elementary functions derived from a model constructed on the basis of some simplifying premises for ordered (either C + H+ + S or C + S + H+) and random reaction mechanisms. In addition, the comparison of the kinetic parameters in fully energized and depolarized cells provided information about the free carrier charge. It was concluded that the binding sequence of formation of the ternary carrier/H+/substrate complex follows a random mechanism and that the carrier bears a negative charge.
在亲脂性阳离子四苯基鏻（TPP+）使质膜选择性去极化的条件下，对 obligatory aerobic酵母粘红酵母中的H+/糖同向转运进行了分析。对照实验表明，这种处理不会损害跨膜ΔpH、细胞能荷以及质膜H+-ATP酶的功能。动力学数据拟合为由基于有序（C + H+ + S或C + S + H+）和随机反应机制的一些简化前提构建的模型推导得出的基本函数。此外，对完全活化和去极化细胞中动力学参数的比较提供了有关自由载体电荷的信息。得出的结论是，三元载体/H+/底物复合物形成的结合顺序遵循随机机制，并且载体带有负电荷。
