甲状腺乳头状癌中E-选择素表达及BRAF状态：与临床病理特征的相关性

E-selectin expression and BRAF status in papillary thyroid carcinomas: Correlation with clinicopathologic features.

作者信息

Miccoli Paolo, Torregrossa Liborio, Borrelli Nicla, Materazzi Gabriele, Cacciato Insilla Andrea, Miccoli Mario, Basolo Fulvio

机构信息

Department of Surgical, Medical, Molecular and Critical Area Pathology, Università degli Studi di Pisa, Pisa, Italy.

出版信息

Surgery. 2014 Dec;156(6):1550-7; discussion 1557-8. doi: 10.1016/j.surg.2014.08.049. Epub 2014 Nov 11.

DOI:10.1016/j.surg.2014.08.049

PMID:25456953

Abstract

BACKGROUND

Cell adhesion molecules, represented by the immunoglobulin family and selectins, play an important role in the progression of cancer. A correlation between selectins and tumor aggressiveness has been demonstrated in several reports.

METHODS

Eighty-eight patients (mean age, 41.0 ± 14 years) with papillary thyroid carcinoma (conventional variant and sized approximately 20 mm) were divided in 2 groups: 41 with encapsulated tumors and 47 with tumors with extrathyroidal extension. E-selectin expression was evaluated by immunohistochemical staining and semiquantitative real-time reverse-transcription polymerase chain reaction and normalized by calculating the z-score (positive: value above the population mean; negative: below the mean).

RESULTS

Lymph node metastasis (LNM) was found in 2 of 41 encapsulated tumors (4.8%) and in 19 of 47 tumors (40.4%) with extrathyroidal extension. BRAF mutation was present in 21 encapsulated tumors (51.2%) and in 31 tumors with extrathyroidal extension (65.9%). The mean E-selectin z-score was -0.32 for encapsulated tumors and 0.28 for tumors with extrathyroidal extension. E-selectin expression correlates with neoplastic infiltration (P = .04), the American Joint Commission on Cancer stage (P = .02), and BRAF mutation (P = .03).

CONCLUSION

E-selectin overexpression in association with BRAF mutation status could promote a more aggressive phenotype in papillary thyroid carcinoma.

摘要

背景

以免疫球蛋白家族和选择素为代表的细胞黏附分子在癌症进展中起重要作用。多项报道证实了选择素与肿瘤侵袭性之间存在相关性。

方法

88例乳头状甲状腺癌患者（平均年龄41.0±14岁，为传统型变异且大小约20mm）被分为两组：41例为包膜内肿瘤患者，47例为甲状腺外侵犯肿瘤患者。通过免疫组织化学染色和半定量实时逆转录聚合酶链反应评估E-选择素表达，并通过计算z值进行标准化（阳性：值高于总体均值；阴性：值低于均值）。

结果

41例包膜内肿瘤中有2例（4.8%）发生淋巴结转移（LNM），47例甲状腺外侵犯肿瘤中有19例（40.4%）发生LNM。21例包膜内肿瘤（51.2%）和31例甲状腺外侵犯肿瘤（65.9%）存在BRAF突变。包膜内肿瘤的平均E-选择素z值为-0.32，甲状腺外侵犯肿瘤的平均E-选择素z值为0.28。E-选择素表达与肿瘤浸润（P = 0.04）、美国癌症联合委员会分期（P = 0.02）和BRAF突变（P = 0.03）相关。