一种用于批量微量热计的停流混合器装置，应用于NAD - NAD酶反应。

A stopped-flow mixer device for a batch microcalorimeter application to NAD-NADase reaction.

作者信息

Berger R L, Mudd C P, Clem T, Kolobow T, Beile E, Simons P C, Michel S, McClintock W

机构信息

National Heart, Lung, and Blood Institute, Laboratory of Technical Development, Bethesda, MD 20892.

出版信息

J Biochem Biophys Methods. 1989 Mar;18(2):113-24. doi: 10.1016/0165-022x(89)90073-0.

DOI:10.1016/0165-022x(89)90073-0

PMID:2545761

Abstract

A new molded polypropylene, diamond-like carbon (DLC)-coated mixing cell has been developed for use in the batch microcalorimeter. Reagent volume can be varied from 25 microliters to 100 microliters. A 10 microcalorie reaction heat can be measured to 5%. Repeat reactions can be done as often as every 10 min for a fast reaction. Reactions can be started within 1 h or less after loading. A pre-equilibrator and a temperature-controlled syringe drive unit permit solutions to be stored at 4 degrees C while being run at any temperature from -20 degrees C to 40 degrees C. The kinetics and enthalpy of reaction of NAD-NADase have been measured. delta H is about 21 kcal/mol endothermic.

摘要

一种新型的模制聚丙烯、类金刚石碳（DLC）涂层混合池已被开发出来，用于批量微热量计。试剂体积可在25微升至100微升之间变化。10微卡路里的反应热可测量至5%。对于快速反应，重复反应可每10分钟进行一次。加载后1小时或更短时间内即可开始反应。预平衡器和温度控制的注射器驱动单元允许溶液在4摄氏度下储存，同时可在-20摄氏度至40摄氏度的任何温度下运行。已测量了NAD - NADase反应的动力学和焓。ΔH约为21千卡/摩尔吸热。