Muller-Steffner H, Muzard M, Oppenheimer N, Schuber F
Laboratoire de Chimie Bioorganique (URA CNRS 1386), Faculté de Pharmacie, Strasbourg-Illkirch, France.
Biochem Biophys Res Commun. 1994 Nov 15;204(3):1279-85. doi: 10.1006/bbrc.1994.2601.
Calf spleen NAD+glycohydrolase, besides its well known reactions, was shown to catalyze hydrolysis and methanolysis (with retention of configuration) of cyclic ADP-ribose, indicating that this classical NADase also belongs to the class of cyclic ADP-ribose hydrolases. No formation of cyclic ADP-ribose could be detected during the hydrolysis of beta-NAD+; moreover, the kinetic parameters of cyclic ADP-ribose seem to rule out that it is a kinetically competent reaction intermediate in the conversion of NAD+ into ADP-ribose by this enzyme.
小牛脾脏NAD⁺糖水解酶，除了其众所周知的反应外，还被证明可催化环ADP - 核糖的水解和甲醇解（构型保持），这表明这种经典的NAD酶也属于环ADP - 核糖水解酶类。在β - NAD⁺水解过程中未检测到环ADP - 核糖的形成；此外，环ADP - 核糖的动力学参数似乎排除了它是该酶将NAD⁺转化为ADP - 核糖过程中的动力学活性反应中间体的可能性。
