结核分枝杆菌（佐夫）莱曼和诺伊曼（ATCC35812）（久野）的完整注释基因组序列

Complete annotated genome sequence of Mycobacterium tuberculosis (Zopf) Lehmann and Neumann (ATCC35812) (Kurono).

作者信息

Miyoshi-Akiyama Tohru, Satou Kazuhito, Kato Masako, Shiroma Akino, Matsumura Kazunori, Tamotsu Hinako, Iwai Hiroki, Teruya Kuniko, Funatogawa Keiji, Hirano Takashi, Kirikae Teruo

机构信息

Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan; Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan.

Okinawa Life Science Research Center, 5-8 Suzaki, Uruma, Okinawa, 904-2234, Japan.

出版信息

Tuberculosis (Edinb). 2015 Jan;95(1):37-9. doi: 10.1016/j.tube.2014.10.007. Epub 2014 Oct 31.

DOI:10.1016/j.tube.2014.10.007

PMID:25458614

Abstract

We report the completely annotated genome sequence of Mycobacterium tuberculosis (Zopf) Lehmann and Neumann (ATCC35812) (Kurono), which is a used for virulence and/or immunization studies. The complete genome sequence of M. tuberculosis Kurono was determined with a length of 4,415,078 bp and a G+C content of 65.60%. The chromosome was shown to contain a total of 4,340 protein-coding genes, 53 tRNA genes, one transfer messenger RNA for all amino acids, and 1 rrn operon. Lineage analysis based on large sequence polymorphisms indicated that M. tuberculosis Kurono belongs to the Euro-American lineage (lineage 4). Phylogenetic analysis using whole genome sequences of M. tuberculosis Kurono in addition to 22 M. tuberculosis complex strains indicated that H37Rv is the closest relative of Kurono based on the results of phylogenetic analysis. These findings provide a basis for research using M. tuberculosis Kurono, especially in animal models.

摘要

我们报告了结核分枝杆菌（祖普夫）莱曼和诺伊曼（ATCC35812）（久野）的完全注释基因组序列，该菌株用于毒力和/或免疫研究。久野结核分枝杆菌的完整基因组序列已确定，长度为4,415,078 bp，G+C含量为65.60%。该染色体共包含4,340个蛋白质编码基因、53个tRNA基因、一个用于所有氨基酸的转移信使RNA和1个rrn操纵子。基于大序列多态性的谱系分析表明，久野结核分枝杆菌属于欧美谱系（谱系4）。除22株结核分枝杆菌复合菌株外，使用久野结核分枝杆菌全基因组序列进行的系统发育分析表明，基于系统发育分析结果，H37Rv是久野最接近的亲缘菌株。这些发现为使用久野结核分枝杆菌进行研究提供了基础，尤其是在动物模型中。