Kanopienė Daiva, Smailytė Giedrė, Vidugirienė Jolanta, Bacher Jeff
National Cancer Institute, Vilnius, Lithuania.
National Cancer Institute, Vilnius, Lithuania.
Medicina (Kaunas). 2014;50(4):216-21. doi: 10.1016/j.medici.2014.09.002. Epub 2014 Sep 30.
Endometrial cancer (EC) is the most commonly diagnosed gynecologic malignancy among women worldwide and may be classified on the basis of different molecular, pathologic and genetic alterations, including microsatellite instability (MSI). Although MSI is associated with a more favorable outcome in colorectal cancer, its relationship with prognosis in EC cancer is not yet clear. The aim of our study is to identify whether MSI correlates with survival of patients in EC.
We examined MSI status and survival of 109 women. MSI was detected by employing the Promega MSI Analysis System, which used 5 mononucleotides markers (BAT-25, BAT-26, NR-21, NR-24, and MONO-27) to identify MSI in a tumor and normal tissue DNA and 2 pentanucleotide markers (Penta C and Penta D) for specimen identification. Median follow-up of patients was 40.4 months (range 5.2-47.9). Survival was estimated by the Kaplan-Meier method and Cox regression analysis was used to assess the effects of different variables on patient survival.
MSI-high was detected in 15.6% EC cases, all of which were associated with endometrioid type histology. Kaplan-Meier survival analysis showed no statistically significant differences between patients with MSI-high and MSI stable tumors (P=0.4) and multivariate analysis concluded that MSI status remained insignificant after stage, histology and tumor grade adjustment (P=0.5).
Our study showed no statistically significant relationship between MSI-high and survival of endometrial cancer patients.
子宫内膜癌（EC）是全球女性中最常被诊断出的妇科恶性肿瘤，可根据不同的分子、病理和基因改变进行分类，包括微卫星不稳定性（MSI）。虽然MSI在结直肠癌中与更有利的预后相关，但其与EC预后的关系尚不清楚。我们研究的目的是确定MSI是否与EC患者的生存率相关。
我们检测了109名女性的MSI状态和生存率。采用Promega MSI分析系统检测MSI，该系统使用5个单核苷酸标记（BAT-25、BAT-26、NR-21、NR-24和MONO-27）来识别肿瘤和正常组织DNA中的MSI，以及2个五核苷酸标记（Penta C和Penta D）用于样本识别。患者的中位随访时间为40.4个月（范围5.2 - 47.9个月）。采用Kaplan-Meier法估计生存率，并使用Cox回归分析评估不同变量对患者生存的影响。
在15.6%的EC病例中检测到高微卫星不稳定性（MSI-high），所有这些病例均与子宫内膜样组织学类型相关。Kaplan-Meier生存分析显示，MSI-high组和微卫星稳定（MSI稳定）肿瘤组患者之间无统计学显著差异（P = 0.4），多变量分析得出，在对分期、组织学和肿瘤分级进行调整后，MSI状态仍无统计学意义（P = 0.5）。
我们的研究表明，MSI-high与子宫内膜癌患者的生存率之间无统计学显著关系。
