Kanopiene Daiva, Vidugiriene Jolanta, Valuckas Konstantinas Povilas, Smailyte Giedre, Uleckiene Saule, Bacher Jeff
Out Patient Clinic, National cancer institute, Santariskiu 1, LT-08660, Vilnius, Lithuania.
Promega Corporation, Madison WI, USA.
Open Med (Wars). 2014 Nov 11;10(1):70-76. doi: 10.1515/med-2015-0005. eCollection 2015.
Microsatellite instability (MSI) is an important factor in the development of various cancers as an identifier of a defective DNA mismatch repair system. The objective of our study was to define the association between microsatellite instability status and traditional clinicopathologic characteristics of endometrioid type adenocarcinoma.
MSI status of endometrial cancer was examined by employing the Promega MSI Analysis System. This system uses 5 mononucleotide markers to identify MSI in tumour and normal tissue DNA (BAT-25, BAT-26, NR-21, NR-24, and MONO-27), and 2 pentanucleotide markers (Penta C and Penta D) for specimen identification. In this study, we investigated MSI status in 109 endometrial carcinomas.
One hundred (92%) of 109 endometrial cancers showed endometrioid type histology and only 9 (8%) non-endometrioid type. MSI-high was found in 17% (17/100) of endometrioid type adenocarcinomas, in 0% (0/9) of non-endometrioid carcinomas. Selected clinicopathologic parameters for endometrioid type adenocarcinomas were compared to the MSI status which was separated into two groups - MSI-high and MSI stable. The results showed that MSI-high status was related to clinicopathologic parameters such as deep myometrial invasion and higher histologic grade in endometrioid type adenocarcinomas.
微卫星不稳定性（MSI）作为DNA错配修复系统缺陷的一个标识，是多种癌症发生发展中的一个重要因素。我们研究的目的是明确微卫星不稳定性状态与子宫内膜样腺癌传统临床病理特征之间的关联。
采用Promega MSI分析系统检测子宫内膜癌的MSI状态。该系统使用5个单核苷酸标记物（BAT-25、BAT-26、NR-21、NR-24和MONO-27）来识别肿瘤和正常组织DNA中的MSI，以及2个五核苷酸标记物（Penta C和Penta D）用于样本识别。在本研究中，我们调查了109例子宫内膜癌的MSI状态。
109例子宫内膜癌中，100例（92%）呈子宫内膜样组织学类型，仅9例（8%）为非子宫内膜样类型。在子宫内膜样腺癌中，17%（17/100）为微卫星高度不稳定（MSI-H），在非子宫内膜样癌中为0%（0/9）。将子宫内膜样腺癌选定的临床病理参数与分为两组的MSI状态（MSI-H和MSI稳定）进行比较。结果显示，MSI-H状态与子宫内膜样腺癌的临床病理参数相关，如肌层深层浸润和较高的组织学分级。
