Chang C P, Vesole D H, Nelson J, Oldstone M B, Stinski M F
Department of Microbiology, University of Iowa, Iowa City 52242.
J Virol. 1989 Aug;63(8):3330-7. doi: 10.1128/JVI.63.8.3330-3337.1989.
A human cytomegalovirus early gene which possesses three temporally regulated promoters is located in the large unique component of the viral genome between 0.054 and 0.064 map units (C.-P. Chang, C.L. Malone, and M.F. Stinski, J. Virol. 63:281-290, 1989). This gene contains a major open reading frame (ORF) located 233 bases downstream of the cap site of an early unspliced RNA. The major ORF predicts a polypeptide of 17 kilodaltons (kDa) which contains a glycoproteinlike signal and anchor domains as well as potential N-glycosylation sites. Antisera were prepared against synthetic peptides derived from amino acid sequences within the major ORF. The antisera detected a viral glycoprotein of 48 kDa in infected cells and recognized the in vitro-translated 17-kDa protein early-gene product. The viral glycoprotein, designated gp48, was modified by N-linked glycans and possibly O-linked glycans. The synthesis of gp48 occurred in the absence of viral DNA replication but accumulated to the highest levels at late times after infection. Since gp48 was found in the virion, it is considered an early structural glycoprotein.
一个具有三个时间调控启动子的人巨细胞病毒早期基因位于病毒基因组的大独特区域，在0.054至0.064个图距单位之间（C.-P. 张、C.L. 马龙和M.F. 斯廷斯基，《病毒学杂志》63:281 - 290，1989年）。该基因包含一个主要开放阅读框（ORF），位于一个早期未剪接RNA帽位点下游233个碱基处。主要ORF预测一个17千道尔顿（kDa）的多肽，其包含一个糖蛋白样信号和锚定结构域以及潜在的N - 糖基化位点。针对从主要ORF内氨基酸序列衍生的合成肽制备了抗血清。抗血清在感染细胞中检测到一种48 kDa的病毒糖蛋白，并识别体外翻译的17 kDa蛋白质早期基因产物。这种病毒糖蛋白命名为gp48，被N - 连接聚糖以及可能的O - 连接聚糖修饰。gp48的合成在没有病毒DNA复制的情况下发生，但在感染后期积累到最高水平。由于在病毒粒子中发现了gp48，它被认为是一种早期结构糖蛋白。
