US Dairy Forage Research Center, Agricultural Research Service, United States Department of Agriculture, Madison, WI 53706, USA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Bioresour Technol. 2015 Jan;175:97-101. doi: 10.1016/j.biortech.2014.10.054. Epub 2014 Oct 18.
Mixed bacterial communities from the rumen ferment cellulosic biomass primarily to C2-C4 volatile fatty acids, and perform only limited chain extension to produce C5 (valeric) and C6 (caproic) acids. The aim of this study was to increase production of caproate and valerate in short-term in vitro incubations. Co-culture of mixed ruminal microbes with a rumen-derived strain of the bacterium Clostridium kluyveri converted cellulosic biomass (alfalfa stems or switchgrass herbage) plus ethanol to VFA mixtures that include valeric and caproic acids as the major fermentation products over a 48-72h run time. Concentrations of caproate reached 6.1gL(-1), similar to or greater than those reported in most conventional carboxylate fermentations that employ substantially longer run times.
瘤胃发酵细胞生物质的混合细菌群落主要将其转化为 C2-C4 挥发性脂肪酸，并且仅进行有限的链延伸以产生 C5（缬草酸）和 C6（己酸）酸。本研究的目的是增加短期体外培养中己酸和戊酸的产量。将混合瘤胃微生物与瘤胃来源的产丁酸梭菌（Clostridium kluyveri）菌株共培养，可将纤维素生物质（紫花苜蓿茎或柳枝稷草料）加乙醇转化为 VFA 混合物，在 48-72h 的运行时间内，该混合物包括作为主要发酵产物的缬草酸和己酸。己酸的浓度达到 6.1g/L(-1)，与大多数采用更长运行时间的常规羧酸发酵中报告的浓度相似或更高。
