Villegas-Rodríguez Sharon B, Arreola-Vargas Jorge, Buitrón Germán
Laboratory for Research On Advanced Processes for Water Treatment, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, 76230, Queretaro, Mexico.
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843, USA.
Environ Sci Pollut Res Int. 2025 Jun;32(28):16617-16626. doi: 10.1007/s11356-024-33103-5. Epub 2024 Apr 1.
Winery effluents containing high ethanol concentrations and diverse organic matter are ideal substrates for producing medium-chain carboxylic acids via fermentation and chain elongation. However, the process needs to be better understood. This study presents novel insights into the bioconversion mechanisms of medium-chain carboxylic acids by correlating fermentation and chain elongation kinetic profiles with the study of microbial communities at different pH (5 to 7) conditions and temperatures (30 to 40 °C). It was found that high productivities of MCCA were obtained using a native culture and winery effluents as a natural substrate. Minor pH variations significantly affected the metabolic pathway of the microorganisms for MCCA production. The maximal productivities of hexanoic (715 mg/L/d) and octanoic (350 mg/L/d) acids were found at pH 6 and 35 °C. Results evidence that the presence of Clostridium, Bacteroides, and Negativicutes promotes the high productions of MCCA. The formation of heptanoic acid was favor when Mogibacterium and Burkholderia were present.
含有高浓度乙醇和多种有机物的葡萄酒厂废水是通过发酵和链延长生产中链羧酸的理想底物。然而，该过程需要被更好地理解。本研究通过将发酵和链延长动力学曲线与不同pH（5至7）条件和温度（30至40°C）下微生物群落的研究相关联，对中链羧酸的生物转化机制提出了新的见解。研究发现，使用天然培养物和葡萄酒厂废水作为天然底物可获得较高的中链羧酸生产率。较小的pH变化显著影响微生物生产中链羧酸的代谢途径。在pH 6和35°C时，己酸（715 mg/L/d）和辛酸（350 mg/L/d）的最大生产率被发现。结果表明，梭菌属、拟杆菌属和厌氧革兰氏阴性菌的存在促进了中链羧酸的高产。当存在瘤胃杆菌属和伯克霍尔德菌属时，庚酸的形成更为有利。
