Palacios J M, O'Dowd B F, Cotecchia S, Hnatowich M, Caron M G, Lefkowitz R J
Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Life Sci. 1989;44(26):2057-65. doi: 10.1016/0024-3205(89)90352-4.
The deduced protein sequences of the mammalian adrenergic receptors (ARs) suggest that these proteins have evolved by several ancient gene duplication events. To investigate in what species these events may have occurred DNA fragments encoding the family of adrenergic receptors from human (beta 1AR and alpha 2AR) and hamster (beta 2AR and alpha 1AR) were used to detect homologous sequences in other vertebrates, invertebrates and unicellular organisms by Southern blot hybridization analysis. Sequences homologous to hamster beta 2AR were detected in lower vertebrates, invertebrates and Dictyostelium, but not in yeast or bacteria. Within vertebrates, sequences strongly homologous to human beta 1AR and human platelet alpha 2AR were confined to the higher vertebrates only. In the invertebrates, only Drosophila contained sequences homologous to hamster alpha 1AR. Our results suggest that non-mammalian species may contain receptors homologous to the mammalian adrenergic receptors and that the sequences homologous to human beta 2AR have been the most strongly conserved.
哺乳动物肾上腺素能受体（ARs）的推导蛋白质序列表明，这些蛋白质是通过几次古老的基因复制事件进化而来的。为了研究这些事件可能发生在哪些物种中，编码人类（β1AR和α2AR）和仓鼠（β2AR和α1AR）肾上腺素能受体家族的DNA片段被用于通过Southern印迹杂交分析检测其他脊椎动物、无脊椎动物和单细胞生物中的同源序列。在低等脊椎动物、无脊椎动物和盘基网柄菌中检测到了与仓鼠β2AR同源的序列，但在酵母或细菌中未检测到。在脊椎动物中，与人类β1AR和人类血小板α2AR高度同源的序列仅局限于高等脊椎动物。在无脊椎动物中，只有果蝇含有与仓鼠α1AR同源的序列。我们的结果表明，非哺乳动物物种可能含有与哺乳动物肾上腺素能受体同源的受体，并且与人类β2AR同源的序列是最保守的。
