Emorine L J, Marullo S, Delavier-Klutchko C, Kaveri S V, Durieu-Trautmann O, Strosberg A D
Department of Biotechnology, Pasteur Institute, Paris, France.
Proc Natl Acad Sci U S A. 1987 Oct;84(20):6995-9. doi: 10.1073/pnas.84.20.6995.
The genomic gene coding for the human beta 2-adrenergic receptor (beta 2AR) from A431 epidermoid cells has been isolated. Transfection of the gene into eukaryotic cells restores a fully active receptor/GTP-binding protein/adenylate cyclase complex with beta 2AR properties. Southern blot analyses with beta 2AR-specific probes show that a single beta 2AR gene is common to various human tissues and that its flanking sequences are highly conserved among humans and between man and rabbit, mouse, and hamster. Functional significance of these regions is supported by the presence of a promoter region (including mRNA cap sites, two "TATA boxes," a "CAAT box," and three G + C-rich regions that resemble binding sites for transcription factor Sp1) 200-300 base pairs 5' to the translation initiation codon. In the 3' flanking region, sequences homologous to glucocorticoid-response elements might be responsible for the increased expression of the beta 2AR gene observed after treatment of the transfected cells with hydrocortisone. In addition, 5' to the promoter region, an open reading frame encodes a 251-residue polypeptide that displays striking homologies with protein kinases and other nucleotide-binding proteins.
已从A431表皮样细胞中分离出编码人β2 - 肾上腺素能受体（β2AR）的基因组基因。将该基因转染到真核细胞中可恢复具有β2AR特性的完全活性的受体/ GTP结合蛋白/腺苷酸环化酶复合物。用β2AR特异性探针进行的Southern印迹分析表明，单个β2AR基因在各种人类组织中普遍存在，并且其侧翼序列在人类之间以及人与兔、小鼠和仓鼠之间高度保守。这些区域的功能意义得到了位于翻译起始密码子5'端200 - 300个碱基对处的启动子区域（包括mRNA帽位点、两个“TATA盒”、一个“CAAT盒”以及三个类似于转录因子Sp1结合位点的富含G + C的区域）的支持。在3'侧翼区域，与糖皮质激素反应元件同源的序列可能是在用氢化可的松处理转染细胞后观察到的β2AR基因表达增加的原因。此外，在启动子区域的5'端，一个开放阅读框编码一个251个残基的多肽，该多肽与蛋白激酶和其他核苷酸结合蛋白具有显著的同源性。
