Edwards J Vincent, Graves Elena, Bopp Alvin, Prevost Nicolette, Santiago Michael, Condon Brian
USDA-ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA.
J Funct Biomater. 2014 Nov 28;5(4):273-87. doi: 10.3390/jfb5040273.
Greige cotton contains waxes and pectin on the outer surface of the fiber that are removed when bleached, but these components present potential wound dressing functionality. Cotton nonwovens blended with hydrophobic and hydrophilic fibers including viscose, polyester, and polypropylene were assessed for clotting activity with thromboelastography (TEG) and thrombin production. Clotting was evaluated based on TEG measurements: R (time to initiation of clot formation), K (time from end of R to a 20 mm clot), α (rate of clot formation according to the angle tangent to the curve as K is reached), and MA (clot strength). TEG values correlate to material surface polarity as measured with electrokinetic parameters (ζplateau, Δζ and swell ratio). The material surface polarity (ζplateau) varied from -22 to -61 mV. K values and thrombin concentrations were found to be inversely proportional to ζplateau with an increase in material hydrophobicity. An increase in the swell ratios of the materials correlated with decreased K values suggesting that clotting rates following fibrin formation increase with increasing material surface area due to swelling. Clot strength (MA) also increased with material hydrophobicity. Structure/function implications from the observed clotting physiology induced by the materials are discussed.
坯布棉纤维外表面含有蜡质和果胶，漂白时会被去除，但这些成分具有潜在的伤口敷料功能。对与包括粘胶、聚酯和聚丙烯在内的疏水和亲水纤维混合的棉无纺布进行了血栓弹力图（TEG）和凝血酶生成的凝血活性评估。基于TEG测量评估凝血情况：R（凝血形成开始时间）、K（从R结束到形成20毫米凝块的时间）、α（达到K时根据曲线切线角度的凝血形成速率）和MA（凝块强度）。TEG值与用电动力学参数（ζ平台、Δζ和溶胀率）测量的材料表面极性相关。材料表面极性（ζ平台）在-22至-61 mV之间变化。发现K值和凝血酶浓度与ζ平台成反比，材料疏水性增加。材料溶胀率的增加与K值降低相关，这表明纤维蛋白形成后的凝血速率随着材料因溶胀而表面积增加而加快。凝块强度（MA）也随着材料疏水性的增加而增加。讨论了材料诱导的观察到的凝血生理的结构/功能意义。
