Research Center for Bioengineering and Sensing Technology, Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, PR China.
Research Center for Bioengineering and Sensing Technology, Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, PR China.
Biosens Bioelectron. 2015 Apr 15;66:155-61. doi: 10.1016/j.bios.2014.10.073. Epub 2014 Nov 13.
This study describes a novel Au nanocluster-based fluorescent sensor for label-free, separation-free and selective detection of cysteamine (CSH). The sensing mechanism is based on CSH etching-induced fluorescence quenching of the bovine serum albumin-protected Au25 nanoclusters (BSAGNCs). A series of characterizations is carried out towards a better understanding of the CSH-induced fluorescence quenching of the BSAGNCs. It is found that CSH can etch the Au25 nanoclusters, exhibiting the potent etching activity. Other thiol-containing compounds such as glutathione and cysteine and other 19 natural amino acids do not interfere with such CSH-induced etching process. The decreases in fluorescence intensity of the BSAGNCs allow sensitive detection of free CSH in the range of 500-10,000nM. The detection limit for CSH is 150nM (S/N=3). The spiked human serum samples can be analyzed with satisfactory results.
本研究描述了一种基于金纳米簇的新型荧光传感器，用于无标记、无需分离且选择性检测半胱胺（CSH）。该传感机制基于牛血清白蛋白保护的 Au25 纳米簇（BSAGNCs）中 CSH 诱导的荧光猝灭。进行了一系列的表征以更好地理解 CSH 诱导的 BSAGNCs 荧光猝灭。结果发现，CSH 可以蚀刻 Au25 纳米簇，表现出很强的蚀刻活性。其他含巯基的化合物，如谷胱甘肽和半胱氨酸以及其他 19 种天然氨基酸，不会干扰这种 CSH 诱导的蚀刻过程。BSAGNCs 的荧光强度降低可在 500-10000nM 范围内实现对游离 CSH 的灵敏检测。CSH 的检测限为 150nM（S/N=3）。可以对加标人血清样品进行分析，结果令人满意。
