Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Calle La Serna, no. 56, CP 24071, León, Spain.
Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, CP 24071, León, Spain.
Sci Total Environ. 2015 Feb 15;506-507:380-90. doi: 10.1016/j.scitotenv.2014.11.039. Epub 2014 Nov 25.
Bacteria are key players in wetland ecosystems, however many essential aspects regarding the ecology of wetland bacterial communities remain unknown. The present study characterizes soil bacterial communities from natural and constructed wetlands through the pyrosequencing of 16S rDNA genes in order to evaluate the influence of wetland variables on bacterial community composition and structure. The results show that the composition of soil bacterial communities was significantly associated with the wetland type (natural or constructed wetland), the type of environment (lagoon, Typha or Salix) and three continuous parameters (SOM, COD and TKN). However, no clear associations were observed with soil pH. Bacterial diversity values were significantly lower in the constructed wetland with the highest inlet nutrient concentrations. The abundances of particular metabolic groups were also related to wetland characteristics.
细菌是湿地生态系统中的关键角色，但许多关于湿地细菌群落生态学的基本方面仍然未知。本研究通过对 16S rDNA 基因进行焦磷酸测序，对天然湿地和人工湿地的土壤细菌群落进行了特征描述，以评估湿地变量对细菌群落组成和结构的影响。结果表明，土壤细菌群落的组成与湿地类型（天然湿地或人工湿地）、环境类型（泻湖、香蒲或柳树）和三个连续参数（SOM、COD 和 TKN）显著相关。然而，与土壤 pH 没有明显的关联。在具有最高入口营养浓度的人工湿地中，细菌多样性值显著较低。特定代谢群的丰度也与湿地特征有关。
