光动力疗法作为常规根管再治疗的附加手段的抗菌效果：一项临床研究。
The antimicrobial effectiveness of photodynamic therapy used as an addition to the conventional endodontic re-treatment: a clinical study.
机构信息
Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Gundulićeva 5, 10 000 Zagreb, Croatia.
Department of Clinical and Molecular Microbiology, University Clinical Hospital Centre, 10 000 Zagreb, Croatia.
出版信息
Photodiagnosis Photodyn Ther. 2014 Dec;11(4):549-55. doi: 10.1016/j.pdpdt.2014.10.004. Epub 2014 Oct 24.
BACKGROUND
The purpose of the study was to evaluate the efficacy of antimicrobial photodynamic therapy (aPDT) used as an adjunct to the endodontic re-treatment in the eradication of microorganisms from previously filled root canals.
METHODS
The study sample consisted of 21 randomly selected patients with root filled and infected root canal system with chronic apical periodontitis on incisors or canines, who have had previously endodontic treatment. Microbiological samples from the root canals were collected after accessing the canal, following the endodontic re-treatment and after the aPDT procedure. During instrumentation, the root canals were irrigated with 2.5% sodium hypochlorite (NaOCl), and the final irrigation protocol included 17% ethylenediaminetetraacetic acid followed by NaOCl. Root canals were filled with a phenothiazinium chloride and irradiated with a diode laser (λ=660 nm, 100 mW) for 1 min. Microbiological samples from the root canals were cultivated on selective plates, and the identification was done by micromorphology, macromorphology and different API strips as well as bacterial counts (colony forming units).
RESULTS
Fourteen bacteria species were isolated from the root canals initially, with a mean value of 4.57 species per canal. Although endodontic re-treatment alone produced a significant reduction in the number of bacteria species (p<0.001), the combination of endodontic treatment and aPDT was statistically more effective (p<0.001). No bacteria were cultivated from the main root canals of 11 teeth.
CONCLUSION
The results indicated that the aPDT used as an adjunct to the conventional endodontic therapy achieved a significant further reduction of intracanal microbial load.
背景
本研究旨在评估抗菌光动力疗法（aPDT）作为根管再治疗辅助手段在消除先前填充的根管内微生物方面的疗效。
方法
研究样本包括 21 名随机选择的前牙或尖牙有慢性根尖周炎的根管再治疗患者，这些患者之前已经接受过根管治疗。在进入根管后、根管再治疗后和 aPDT 后，从根管中采集微生物样本。在器械处理过程中，用 2.5%次氯酸钠（NaOCl）冲洗根管，最后冲洗方案包括 17%乙二胺四乙酸（EDTA）继之以 NaOCl。用 phenothiazinium 氯化物填充根管，并使用二极管激光（λ=660nm，100mW）照射 1 分钟。从根管中采集微生物样本，接种于选择性平板上，通过微观形态学、宏观形态学和不同 API 条带以及细菌计数（菌落形成单位）进行鉴定。
结果
最初从根管中分离出 14 种细菌，平均每个根管有 4.57 种细菌。尽管单独进行根管再治疗已显著减少了细菌种类的数量（p<0.001），但根管治疗联合 aPDT 的效果更为显著（p<0.001）。11 颗主根管中未培养出细菌。
结论
结果表明，aPDT 作为常规根管治疗的辅助手段，可显著进一步降低根管内微生物负荷。
Zotero 插件