建立一种用于同时检测肉产品中鸡、鸭和鹅 DNA 的敏感且特异的多重 PCR 方法。
Development of a sensitive and specific multiplex PCR method for the simultaneous detection of chicken, duck and goose DNA in meat products.
机构信息
National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China.
National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China.
出版信息
Meat Sci. 2015 Mar;101:90-4. doi: 10.1016/j.meatsci.2014.11.007. Epub 2014 Nov 22.
Identifying the origin of animal species in manufactured meat products is of considerable economic, religious and sanitary importance. In this study, we developed a multiplex PCR method to simultaneously detect chicken, duck and goose DNA in meat products derived from beef, pork, mutton or quail. The PCR primers were designed based on the sequence of mitochondrial genes of each avian species, and the amplicon sizes were 131, 283 and 387bp for chicken, duck and goose, respectively. The method had no cross-reaction with DNA isolated from beef, mutton, pork or quail, and generated products at a target DNA content as low as 0.05ng, or a target meat content of 1% of total meat weight. Moreover, screening of 24 commercial meat samples using this method indicated that six, two and one samples were contaminated with chicken, duck, or both, respectively, suggesting its usefulness for the simultaneous identification of chicken, duck and goose DNA in commercial meat products.
鉴定动物物种在加工肉品中的来源具有重要的经济、宗教和卫生意义。在本研究中，我们开发了一种多重 PCR 方法，可同时检测来源于牛肉、猪肉、羊肉或鹌鹑的肉品中的鸡、鸭和鹅 DNA。PCR 引物是根据每种禽类的线粒体基因序列设计的，扩增子大小分别为鸡、鸭和鹅的 131、283 和 387bp。该方法与从牛肉、羊肉、猪肉或鹌鹑中提取的 DNA 无交叉反应，在目标 DNA 含量低至 0.05ng 或目标肉含量为总肉重的 1%时即可产生产物。此外，使用该方法对 24 个商业肉样进行筛选，结果表明，六个、两个和一个样品分别受到鸡、鸭或两者的污染，这表明该方法可用于同时鉴定商业肉品中的鸡、鸭和鹅 DNA。
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