Liu Guo-Qiang, Luo Jian-Xing, Xu Wei-Liang, Li Chun-Dong, Guo Yuan-Sheng, Guo Liang
Xilin Gol Food Testing and Risk Assessment Center Xilin Gol Institute of Bioengineering Xilingol Vocational College Xilinhot China.
Food Sci Nutr. 2021 Apr 2;9(6):3130-3141. doi: 10.1002/fsn3.2272. eCollection 2021 Jun.
The authentication and labeling of meat products, concerning origins and species, are key to fair trade and to protect consumer interests in the market. We developed an improved triplex real-time PCR approach to simultaneously identify chicken, duck, and goose DNA in meat, including an endogenous control to avoid false negatives. Our method specifically detected DNA from chicken, duck, and goose, and showed no cross-reaction with DNA extracted from other meat types. The detection limits of chicken, duck, and goose DNA were 0.001-0.00025 ng, 0.0025-0.0001 ng, and 0.001-0.00001 ng, respectively, and we were able to simultaneously identify DNA from two distinct origins using as little as 0.1% of total meat weight. Our newly generated triplex real-time PCR method with endogenous control exhibited high specificity, sensitivity, and efficiency for simultaneous identification of DNA from chicken, duck, and goose in meat.
肉类产品的产地和物种鉴定及标签标注是公平贸易以及保护市场中消费者利益的关键所在。我们开发了一种改进的三重实时荧光定量PCR方法，可同时鉴定肉类中的鸡、鸭和鹅DNA，包括一个内源性对照以避免假阴性。我们的方法能够特异性地检测鸡、鸭和鹅的DNA，且与从其他肉类中提取的DNA无交叉反应。鸡、鸭和鹅DNA的检测限分别为0.001 - 0.00025 ng、0.0025 - 0.0001 ng和0.001 - 0.00001 ng，并且我们能够使用低至总肉重0.1%的样本同时鉴定来自两个不同产地的DNA。我们新开发的带有内源性对照的三重实时荧光定量PCR方法在同时鉴定肉类中鸡、鸭和鹅的DNA时具有高特异性、高灵敏度和高效性。
