即使是自由基也应该遵循一些规则：自由基研究术语和方法学指南。
Even free radicals should follow some rules: a guide to free radical research terminology and methodology.
机构信息
University of California at Merced, Merced, CA 95344, USA; Ethel Percy Andrus Gerontology Center, Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089, USA.
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
出版信息
Free Radic Biol Med. 2015 Jan;78:233-5. doi: 10.1016/j.freeradbiomed.2014.10.504. Epub 2014 Oct 23.
Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species.
自由基和氧化剂现在与生理反应和多种疾病有关。鉴于自由基研究人员的广泛专业知识，对化学的更深入理解并未被普遍应用于生物学研究。我们认为，一些广泛使用的方法和术语阻碍了进展，需要加以解决。我们提出放弃和明智使用几种方法和术语的理由，并提出实际可行的替代方法。这些变化在四个方面提出：使用荧光染料来鉴定和定量活性物质，在复杂生物系统中测量脂质过氧化的方法，抗氧化剂作为自由基清除剂的说法，以及使用活性物质的术语。
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