Edwards S L, Ulrich D, White J F, Su K, Rosamilia A, Ramshaw J A M, Gargett C E, Werkmeister J A
CSIRO Manufacturing Flagship, Bayview Avenue, Clayton, Victoria 3168, Australia.
Department of Obstetrics and Gynaecology, Monash University, Clayton, Victoria 3800, Australia; The Ritchie Centre, MIMR-PHI Institute of Medical Research, 27-31 Wright Street, Clayton, Victoria 3168, Australia.
Acta Biomater. 2015 Feb;13:286-94. doi: 10.1016/j.actbio.2014.10.043. Epub 2014 Nov 21.
Use of synthetic clinical meshes in pelvic organ prolapse (POP) repair can lead to poor mechanical compliance in vivo, as a result of a foreign body reaction leading to excessive scar tissue formation. Seeding mesh with mesenchymal stem cells (MSCs) prior to implantation may reduce the foreign body reaction and lead to improved biomechanical properties of the mesh-tissue complex. This study investigates the influence of seeding human endometrial mesenchymal stem cells (eMSCs) on novel gelatin-coated polyamide scaffolds, to identify differences in scaffold/tissue biomechanical properties and new tissue growth following up to 90 days' implantation, in a subcutaneous rat model of wound repair. Scaffolds were subcutaneously implanted, either with or without eMSCs, in immunocompromised rats and following 7, 30, 60 and 90 days were removed and assessed for their biomechanical properties using uniaxial tensile testing. Following 7, 30 and 90 days' implantation scaffolds were assessed for tissue ingrowth and organization using histological staining and scanning electron microscopy. The eMSCs were associated with altered collagen growth and organization around the mesh filaments of the scaffold, affecting the physiologically relevant tensile properties of the scaffold-tissue complex, in the toe region of the load-elongation curve. Scaffolds seeded with eMSCs were significantly less stiff on initial stretching than scaffolds implanted without eMSCs. Collagen growth and organization were enhanced in the long-term in eMSC-seeded scaffolds, with improved fascicle formation and crimp configuration. Results suggest that neo-tissue formation and remodelling may be enhanced through seeding scaffolds with eMSCs.
在盆腔器官脱垂（POP）修复中使用合成临床网片，由于异物反应导致过度瘢痕组织形成，可能会导致体内机械顺应性较差。在植入前用间充质干细胞（MSCs）接种网片，可能会减少异物反应，并改善网片-组织复合物的生物力学性能。本研究调查了接种人子宫内膜间充质干细胞（eMSCs）对新型明胶包被聚酰胺支架的影响，以确定在伤口修复的皮下大鼠模型中，植入长达90天后支架/组织生物力学性能和新组织生长的差异。将支架皮下植入免疫受损大鼠体内，植入有或没有eMSCs的支架，在7、30、60和90天后取出，使用单轴拉伸试验评估其生物力学性能。在植入7、30和90天后，使用组织学染色和扫描电子显微镜评估支架的组织长入和组织情况。在负载-伸长曲线的起始区域，eMSCs与支架网丝周围胶原生长和组织的改变有关，影响了支架-组织复合物的生理相关拉伸性能。接种eMSCs的支架在初始拉伸时的硬度明显低于未接种eMSCs植入的支架。长期来看，接种eMSCs的支架中胶原生长和组织得到增强，束状结构和卷曲形态得到改善。结果表明，通过接种eMSCs的支架可能会增强新组织形成和重塑。
