Jamuna Rani A, Mythili S V, Nagarajan Shunmugam
Indian J Physiol Pharmacol. 2014 Jan-Mar;58(1):13-6.
Type 2 Diabetes Mellitus (T 2 D M) occurring as a result of reduced insulin action, is seen in a larger section of population. It is also a condition of Oxidative Stress utilizing the antioxidant resources of the body. One such antioxidant is the Paraoxonasel (PON1) enzyme associated with High Density Lipoprotein. So, the activity of PON1 may be reduced in T 2 D M. Hence, this study was taken up to analyze the status of PON I activity in patients with Type 2 Diabetes Mellitus, attending the Diabetic OP of Sree Balaji Medical College and Hospital (SBMCH). The study included 93 Type 2 Diabetic patients and 89 age and sex matched healthy controls. Paraoxonase 1 activity was assayed by Fluorimetry using the Invitrogen Molecular probes kit. There was a significant reduction in PON1 activity (p value > 0.001) along with a decrease in HDL cholesterol among the Type 2 D M patients compared to healthy controls. The progression of Diabetes Mellitus through the years reflected in a much more reduction in PON1 activity as shown by the Pearsons' correlation analysis. The results were analyzed using SPSS statistical package. It is concluded that Type 2 D M being a condition of Oxidative stress has resulted in the reduction of the antioxidant activity of enzyme PON1.
2型糖尿病（T2DM）是由于胰岛素作用降低所致，在较大一部分人群中可见。它也是一种利用体内抗氧化资源的氧化应激状态。一种这样的抗氧化剂是与高密度脂蛋白相关的对氧磷酶1（PON1）酶。因此，T2DM患者中PON1的活性可能降低。因此，本研究旨在分析在斯里·巴拉吉医学院和医院（SBMCH）糖尿病门诊就诊的2型糖尿病患者中PON1活性的状况。该研究包括93名2型糖尿病患者和89名年龄和性别匹配的健康对照者。使用Invitrogen分子探针试剂盒通过荧光法测定对氧磷酶1活性。与健康对照者相比，2型糖尿病患者中PON1活性显著降低（p值>0.001），同时高密度脂蛋白胆固醇降低。多年来糖尿病的进展反映在PON1活性的更大程度降低上，如皮尔逊相关性分析所示。使用SPSS统计软件包分析结果。得出的结论是，2型糖尿病作为一种氧化应激状态导致了对氧磷酶1酶的抗氧化活性降低。
