PathWest Laboratory Medicine WA, Nedlands, Western Australia, Australia.
PathWest Laboratory Medicine WA, Nedlands, Western Australia, Australia; School of Pathology and Laboratory Medicine, University of Western Australia, Nedlands, Australia.
J Clin Virol. 2014 Dec;61(4):579-84. doi: 10.1016/j.jcv.2014.10.018. Epub 2014 Nov 4.
Intussusception, a condition where one segment of intestine invaginates into another, occurs predominantly in infants and young children. A number of potential causes have been identified including infectious agents and rotavirus vaccination. Following the introduction of rotavirus vaccination of infants in Western Australia, a laboratory surveillance programme testing notified intussusception cases for infectious agents was commenced. This led to a PCR-based study of the association between gastrointestinal viruses and intussusception.
Conduct viral testing on stool samples from intussusception patients to determine viruses that may have an association with intussusception.
A retrospective case-control study was conducted using stool samples collected from children with intussusception (n=74) and matched controls (n=289) between 2008 and 2011. Samples were tested for rotavirus, norovirus, adenovirus, enterovirus, rhinovirus, astrovirus, parechovirus and bocavirus. Adenovirus, enterovirus and rhinovirus species were determined by DNA sequencing.
Human adenovirus C was detected in significantly more cases than controls with 31/74 (41.9%) cases testing positive compared to 39/289 (13.49%) controls (OR=4.38, p<0.001). A significant difference was seen in Enterovirus B detections with 11/74 (14.9%) cases testing positive compared to 21/289 (7.3%) controls (OR=2.24, p=0.04). Rotavirus was detected in 7/74 (9.46%) cases and 11/289 (3.81%) controls, which was also a significant difference (OR=2.88, p=0.045).
Our results show that intussusception is associated with non-enteric adenovirus infections, and Enterovirus B infections. While a statistical association was seen with rotavirus and intussusception, we were not able to determine if this was related to vaccine strain or wild type rotavirus.
肠套叠是一种肠段套入另一段肠腔的疾病，主要发生在婴儿和幼儿中。已经确定了一些潜在的原因，包括感染性病原体和轮状病毒疫苗接种。在西澳大利亚为婴儿接种轮状病毒疫苗后，开始了一项实验室监测计划，对报告的肠套叠病例进行感染性病原体检测。这导致了一项基于 PCR 的研究，以确定胃肠道病毒与肠套叠之间的关联。
对肠套叠患者的粪便样本进行病毒检测，以确定可能与肠套叠相关的病毒。
这是一项回顾性病例对照研究，使用 2008 年至 2011 年间收集的肠套叠患儿（n=74）和匹配对照（n=289）的粪便样本进行。样本检测轮状病毒、诺如病毒、腺病毒、肠道病毒、鼻病毒、星状病毒、副流感病毒和博卡病毒。通过 DNA 测序确定腺病毒、肠道病毒和鼻病毒的种类。
与对照组相比，检测到更多的人腺病毒 C，74 例病例中有 31 例（41.9%）检测为阳性，而 289 例对照中有 39 例（13.49%）检测为阳性（比值比=4.38，p<0.001）。肠道病毒 B 的检测也存在显著差异，74 例病例中有 11 例（14.9%）检测为阳性，而 289 例对照中有 21 例（7.3%）检测为阳性（比值比=2.24，p=0.04）。轮状病毒在 7/74（9.46%）例病例和 11/289（3.81%）例对照中被检测到，这也是一个显著差异（比值比=2.88，p=0.045）。
我们的结果表明，肠套叠与非肠道腺病毒感染和肠道病毒 B 感染有关。虽然轮状病毒与肠套叠之间存在统计学关联，但我们无法确定这是否与疫苗株或野生型轮状病毒有关。
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