Wasyl Dariusz, Kern-Zdanowicz Izabela, Domańska-Blicharz Katarzyna, Zając Magdalena, Hoszowski Andrzej
Department of Microbiology, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland.
Vet Microbiol. 2015 Jan 30;175(1):85-91. doi: 10.1016/j.vetmic.2014.10.014. Epub 2014 Oct 24.
Multidrug resistant Salmonella Kentucky strains have been isolated from turkeys in Poland since 2009. Multiple mutations within chromosomal genes gyrA and parC were responsible for high-level ciprofloxacin resistance. One of the isolates was extended spectrum β-lactamase- (ESBL) positive: the strain 1643/2010 carried a conjugative 167,779 bps plasmid of IncA/C family. The sequence analysis revealed that it carried a blaCTX-M-25 gene and an integron with another β-lactamase encoding gene-blaOXA-21. This is the first known report of a CTX-M-25 encoding gene both in Poland and in Salmonella Kentucky world-wide, as well as in the IncA/C plasmid. Analysis of the integron showed a novel arrangement of gene cassettes-aacA4, aacC-A1 and blaOXA-21 where the latter might result from an intergeneric gene transfer. The study confirmed Salmonella Kentucky population isolated in Poland belongs to global epidemics of high level fluoroquinolone resistant clone ST198 that can carry rare β-lactamase genes.
自2009年以来，波兰已从火鸡中分离出耐多药肯塔基沙门氏菌菌株。染色体基因gyrA和parC内的多个突变导致了对环丙沙星的高水平耐药性。其中一株分离株为超广谱β-内酰胺酶（ESBL）阳性：菌株1643/2010携带一个IncA/C家族的167,779 bps接合质粒。序列分析表明，它携带blaCTX-M-25基因和一个带有另一个β-内酰胺酶编码基因blaOXA-21的整合子。这是波兰以及全球肯塔基沙门氏菌中首次发现编码CTX-M-25的基因，也是在IncA/C质粒中首次发现。对整合子的分析显示了基因盒的一种新排列——aacA4、aacC-A1和blaOXA-21，其中后者可能源于基因间转移。该研究证实，在波兰分离出的肯塔基沙门氏菌群体属于耐高水平氟喹诺酮克隆ST198的全球流行株，该克隆可能携带罕见的β-内酰胺酶基因。
