Chen K, Swartz H M
Department of Physiology and Biophysics College of Medicine, University of Illinois, Urbana.
Biochim Biophys Acta. 1989 Jul 21;992(1):131-3. doi: 10.1016/0304-4165(89)90060-3.
The use of nitroxides in functional biological systems has increased greatly as it has become evident that such studies can provide valuable biophysical and metabolic data. This has led to a need to understand the nature of the metabolism of nitroxides and their products. This paper presents data indicating the value of 15N-perdeuterated Tempone specifically to indicate the amount of hydroxylamines that are present in a cellular system. Using this technique, we found that in the mammalian cells that we studied the principal or only products of reduction of doxyl stearates were the corresponding hydroxylamines.
