具有强吞噬活性的爱泼斯坦-巴尔病毒转化细胞系的建立与鉴定

Establishment and characterization of an Epstein-Barr virus transformed cell line with strong phagocytic activity.

作者信息

von den Driesch P, Bhardwaj R, Flad H D, Neugebauer D C, Pielken H J, Urbanitz D, Kölsch E

机构信息

Institut für Immunologie, Universität Münster, West Germany.

出版信息

Blood. 1989 Jul;74(1):430-6.

PMID:2546624

Abstract

An immunoglobulin M (IgM)-positive cell line, Ms 28, apparently spontaneously transformed by Epstein-Barr virus (EBV) was established from peripheral blood cells of a patient with immature myeloblastic leukemia. It has been characterized according to phenotype, cytochemistry, and membrane antigen pattern. The cell line expresses lymphoid markers like CD 19, CD 22, and CD 30 and synthesizes and secretes IgM. Monocyte markers CD 11c, CD 14, and CD 15 are absent. Neither interleukin-1 (IL-1), nor tumor necrosis factor (TNF-alpha) are produced. But Ms 28 cells show strong phagocytic activity and engulf Latex particles and sheep RBCs (SRBCs) that need not to be opsonized. The phagocytic activity can be inhibited by chloroquine. Both phagocytosis and EBV nuclear-antigen (EBNA) expression can be observed in one and the same cell. Ms 28 cells might be useful to study immunologic activities like antigen processing and presentation.

摘要

从一名未成熟髓细胞白血病患者的外周血细胞中建立了一种免疫球蛋白M（IgM）阳性细胞系Ms 28，它显然是由爱泼斯坦-巴尔病毒（EBV）自发转化而来。已根据表型、细胞化学和膜抗原模式对该细胞系进行了表征。该细胞系表达CD 19、CD 22和CD 30等淋巴样标志物，并合成和分泌IgM。单核细胞标志物CD 11c、CD 14和CD 15缺失。既不产生白细胞介素-1（IL-1），也不产生肿瘤坏死因子（TNF-α）。但Ms 28细胞表现出较强的吞噬活性，能吞噬乳胶颗粒和绵羊红细胞（SRBC），且无需调理素。吞噬活性可被氯喹抑制。吞噬作用和EBV核抗原（EBNA）表达可在同一个细胞中观察到。Ms 28细胞可能有助于研究抗原加工和呈递等免疫活性。