Geraldo-Martins Vinícius Rangel, Lepri Cesar Penazzo, Faraoni-Romano Juliana Jendiroba, Palma-Dibb Regina Guenka
Department of Biomaterials, School of Dentistry, Uberaba University, Uberaba, MG, Brazil.
Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, SP, Brazil.
J Appl Oral Sci. 2014 Sep-Oct;22(5):459-64. doi: 10.1590/1678-775720130570.
The use of erbium lasers to prevent caries in enamel has shown positive results. However, it is not known if Er,Cr:YSGG laser can also be used to increase acid resistance of root dentine, which is another dental tissue susceptible to the action of cariogenic bacteria.
To analyze the effects of the Er,Cr:YSGG laser (λ=2.78 μm, 20 Hz) irradiation associated with 2% neutral sodium fluoride (NaF) to prevent root dentin demineralization.
One hundred human root dentin samples were divided into 10 groups (G) and treated as follows: G1: no treatment; G2: NaF; G3: laser (4.64 J/cm2) with water cooling (WC=5.4 mL/min); G4: laser (4.64 J/cm2) without WC; G5: laser (8.92 J/cm2) with WC; G6: laser (8.92 J/cm2) without WC; G7: laser (4.64 J/cm2) with WC and NaF; G8: laser (4.64 J/cm2) without WC and NaF; G9: laser (8.92 J/cm2) with WC and NaF; G10: laser (8.92 J/cm2) without WC and NaF. The NaF gel was applied alone or after 4 min of irradiation. After 14 days of acid challenge, the samples were sectioned and the Knoop microhardness (KHN) test was done at different depths (30, 60, 90 and 120 μm) from the outer dentin surface. Data were analyzed by one-way ANOVA and Fisher's test (α=5%).
The results showed that G8 and G10 presented higher KHN than the G1 for the depths of 30 and 60 μm, indicating an increase of the acid resistance of the dentin in up to 35% (p<0.05).
The use of Er,Cr:YSGG laser irradiation at 4.64 J/ cm2 and 8.92 J/cm2 without water cooling and associated with 2% NaF can increase the acid resistance of human root dentin.
使用铒激光预防牙釉质龋已显示出积极效果。然而,尚不清楚铒铬钇钪镓石榴石(Er,Cr:YSGG)激光是否也可用于提高牙根牙本质的抗酸性,牙根牙本质是另一种易受致龋菌作用影响的牙体组织。
分析铒铬钇钪镓石榴石(Er,Cr:YSGG)激光(波长λ = 2.78μm,频率20Hz)联合2%中性氟化钠(NaF)照射对预防牙根牙本质脱矿的效果。
将100个人类牙根牙本质样本分为10组(G),并进行如下处理:G1:未处理;G2:NaF处理;G3:激光(4.64J/cm²)照射并水冷(WC = 5.4mL/min);G4:激光(4.64J/cm²)照射且无水冷;G5:激光(8.92J/cm²)照射并水冷;G6:激光(8.92J/cm²)照射且无水冷;G7:激光(4.64J/cm²)照射并水冷且使用NaF;G8:激光(4.64J/cm²)照射且无水冷并使用NaF;G9:激光(8.92J/cm²)照射并水冷且使用NaF;G10:激光(8.92J/cm²)照射且无水冷并使用NaF。NaF凝胶单独应用或在照射4分钟后应用。经过14天的酸蚀试验后,将样本切片,并在距牙本质外表面不同深度(30、60、90和120μm)处进行努氏显微硬度(KHN)测试。数据采用单因素方差分析和费舍尔检验(α = 5%)进行分析。
结果显示,在30和60μm深度处,G8和G10组的KHN值高于G1组,表明牙本质的抗酸性提高了35%(p<0.05)。
使用4.64J/cm²和8.92J/cm²的铒铬钇钪镓石榴石(Er,Cr:YSGG)激光照射且无水冷并联合2% NaF可提高人类牙根牙本质的抗酸性。