Tu Meng-Che, Chen Han-Yi, Wang Yuxi, Moochhala Shabbir M, Alagappan Palaniappan, Liedberg Bo
School of Materials Science and Engineering, Nanyang Technological University, Nanyang Avenue, 639798, Singapore; Center for Biomimetic Sensor Science, 50 Nanyang Drive, 637553, Singapore.
School of Materials Science and Engineering, Nanyang Technological University, Nanyang Avenue, 639798, Singapore; Tum Create, 1 Create Way #10-02 Create Tower, 138602, Singapore.
Anal Chim Acta. 2015 Jan 1;853:228-233. doi: 10.1016/j.aca.2014.09.050. Epub 2014 Oct 5.
This article reports on carbon nanotube/manganese dioxide (CNT-MnO2) composites as electrochemical tags for non-enzymatic signal amplification in immunosensing. The synthesized CNT-MnO2 composites showed good electrochemical activity, electrical conductivity and stability. The electrochemical signal of CNT-MnO2 composites coated glassy carbon electrode (GCE) increased by nearly two orders of magnitude compared to bare GCE in hydrogen peroxide (H2O2) environment. CNT-MnO2 composite was subsequently validated as electrochemical tags for sensitive detection of α-fetoprotein (AFP), a tumor marker for diagnosing hepatocellular carcinoma. The electrochemical immunosensor demonstrated a linear response on a log-scale for AFP concentrations ranging from 0.2 to 100 ng mL(-1). The limit of detection (LOD) was estimated to be 40 pg mL(-1) (S/N=3) in PBS buffer. Further measurements using AFP spiked plasma samples revealed the applicability of fabricated CNT-MnO2 composites for clinical and diagnostic applications.
本文报道了碳纳米管/二氧化锰（CNT-MnO₂）复合材料作为免疫传感中非酶信号放大的电化学标记物。合成的CNT-MnO₂复合材料表现出良好的电化学活性、导电性和稳定性。在过氧化氢（H₂O₂）环境中，与裸玻碳电极（GCE）相比，涂覆有CNT-MnO₂复合材料的玻碳电极的电化学信号增加了近两个数量级。随后，CNT-MnO₂复合材料被验证为用于灵敏检测甲胎蛋白（AFP）的电化学标记物，AFP是诊断肝细胞癌的一种肿瘤标志物。该电化学免疫传感器对AFP浓度在0.2至100 ng mL⁻¹范围内呈对数尺度的线性响应。在磷酸盐缓冲盐溶液（PBS）缓冲液中，检测限（LOD）估计为40 pg mL⁻¹（S/N = 3）。使用添加了AFP的血浆样本进行的进一步测量表明，所制备的CNT-MnO₂复合材料适用于临床和诊断应用。
