Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, China.
J Colloid Interface Sci. 2010 May 15;345(2):174-80. doi: 10.1016/j.jcis.2010.01.033. Epub 2010 Feb 6.
A new amperometric immunosensor for the determination of α-1-fetoprotein (AFP) has been constructed. First, a multiwalled carbon nanotube-silver (MWNT-Ag) composite was modified on the surface of a glassy carbon electrode. Then, chitosan-MnO(2) (CS-MnO(2)) with excellent film forming ability was dipped onto the MWNT-Ag-modified electrode. Subsequently, gold nanoparticles were electrodeposited on the electrode to immobilize anti-AFP. The assembly processes were characterized with cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. MWNT-Ag composite was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, and UV-vis spectroscopy. The system was optimized to realize a reliable determination of AFP in the range of 0.25-250 ng/ml with a detection limit of 0.08 ng/ml (S/N=3). The proposed immunosensor showed a rapid and highly sensitive amperometric response to AFP with acceptable stability and reproducibility.
一种用于测定甲胎蛋白（AFP）的新的安培免疫传感器已经构建。首先，在玻碳电极表面修饰多壁碳纳米管-银（MWNT-Ag）复合材料。然后，具有优异成膜能力的壳聚糖-二氧化锰（CS-MnO2）被浸入MWNT-Ag 修饰的电极上。随后，在电极上电沉积金纳米粒子以固定抗 AFP。通过循环伏安法、电化学阻抗谱和扫描电子显微镜对组装过程进行了表征。MWNT-Ag 复合材料通过透射电子显微镜、X 射线光电子能谱和紫外可见光谱进行了表征。该体系被优化，以实现在 0.25-250ng/ml 范围内对 AFP 的可靠测定，检测限为 0.08ng/ml（S/N=3）。所提出的免疫传感器对 AFP 具有快速且高灵敏度的安培响应，具有良好的稳定性和重现性。
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