Zhao Tong, Guan Xiujuan, Tang Wanjin, Ma Ying, Zhang Haixia
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Anal Chim Acta. 2015 Jan 1;853:668-675. doi: 10.1016/j.aca.2014.10.019. Epub 2014 Oct 16.
A kind of new temperature sensitive molecularly imprinted polymer (MIP) with ofloxacin (OFL) as template was prepared for the coating of solid phase microextraction (SPME). Dopamine was self-polymerized on stainless steel fiber (SSF) as the SPME support followed by silanization. Then MIP was synthesized as SPME coating on the modified SSF in a capillary, with N-isopropyl acrylamide as temperature sensitive monomer and methacrylic acid as functional monomer. The synthesis could be well repeated with multiple capillaries putting in the same reaction solution. The obtained MIP fiber was evaluated in detail with different techniques and various adsorption experiments. At last the MIP fiber was used to extract the OFL in milk. Satisfied recoveries between 89.7 and 103.4% were obtained with the limit of quantification (LOQLC) of 0.04 μg mL(-1) by the method of SPME coupled with high performance of liquid chromatography (HPLC).
制备了一种以氧氟沙星（OFL）为模板的新型温敏分子印迹聚合物（MIP），用于固相微萃取（SPME）涂层。多巴胺在不锈钢纤维（SSF）上自聚合作为SPME载体，随后进行硅烷化处理。然后，以N-异丙基丙烯酰胺为温敏单体、甲基丙烯酸为功能单体，在毛细管中于改性SSF上合成MIP作为SPME涂层。将多个毛细管置于同一反应溶液中，该合成过程可良好重复。采用不同技术和各种吸附实验对所得MIP纤维进行了详细评估。最后，将MIP纤维用于提取牛奶中的OFL。通过固相微萃取结合高效液相色谱（HPLC）方法，获得了89.7%至103.4%的满意回收率，定量限（LOQLC）为0.04 μg mL⁻¹。
