Li Xiufang, Liu Hui, Deng Zhiwei, Chen Wenqing, Li Tianhao, Zhang Yunshan, Zhang Zhuomin, He Yao, Tan Zhijian, Zhong Shian
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Polymers (Basel). 2020 Mar 2;12(3):536. doi: 10.3390/polym12030536.
Novel PEGylated thermo-sensitive bionic magnetic core-shell structure molecularly imprinted polymers (PMMIPs) for the specific adsorption and separation of bovine serum albumin (BSA) were obtained via a surface-imprinting technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), fourier transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TGA), and specific surface area (BET), were adopted to demonstrate that novel PMMIPs were successfully synthesized. Subsequently, the prepared PMMIPs were used as the extractor for BSA and were combined with magnetic solid-phase extraction. The concentrations of BSA were detected by UV-vis spectrophotometry at 278 nm. The maximum adsorption capacity of the PMMIPs was 258 mg g, which is much higher than that of non-imprinted polymer (PMNIPs). PMMIPs showed favorable selectivity for BSA against reference proteins, i.e., bovine hemoglobin, ovalbumin and lysozyme. PMMIPs were further used to recognize BSA in protein mixtures, milk, urine and sewage, these results revealed that approximately 96% of the ideal-state adsorption capacity of PMMIPs for BSA was achieved under complicated conditions. Regeneration and reusability studies demonstrated that adsorption capacity loss of the PMMIPs was not obvious after recycling for four times. Facile synthesis, excellent adsorption property and efficient selectivity for BSA trapping are features that highlight PMMIPs as an attractive candidate for biomacromolecular purification.
通过表面印迹技术制备了用于特异性吸附和分离牛血清白蛋白（BSA）的新型聚乙二醇化热敏仿生磁核壳结构分子印迹聚合物（PMMIPs）。采用X射线衍射（XRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、振动样品磁强计（VSM）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）和比表面积（BET）等手段，证明成功合成了新型PMMIPs。随后，将制备的PMMIPs用作BSA的萃取剂，并与磁性固相萃取相结合。通过紫外可见分光光度法在278 nm处检测BSA的浓度。PMMIPs的最大吸附容量为258 mg/g，远高于非印迹聚合物（PMNIPs）。PMMIPs对BSA相对于参考蛋白质（即牛血红蛋白、卵清蛋白和溶菌酶）表现出良好的选择性。PMMIPs进一步用于识别蛋白质混合物、牛奶、尿液和污水中的BSA，这些结果表明，在复杂条件下，PMMIPs对BSA的吸附容量达到了理想状态的约96%。再生和可重复使用性研究表明，PMMIPs在循环使用四次后吸附容量损失不明显。简便的合成方法、优异的吸附性能和对BSA捕获的高效选择性是PMMIPs作为生物大分子纯化有吸引力候选物的突出特点。
