González-Clemente C, Luengo J M, Rodríguez-Aparicio L B, Reglero A
Department of Biochemistry and Molecular Biology, University of León, Campus de Vegazana, Spain.
FEBS Lett. 1989 Jul 3;250(2):429-32. doi: 10.1016/0014-5793(89)80770-7.
Synthesis of colominic acid in Escherichia coli K-235 is strictly regulated by temperature. Evidence for the role of cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) synthetase in this regulation was obtained by measuring its level in E. coli grown at 20 and 37 degrees C. No activity was found in E. coli grown at 20 degrees C. CMP-Neu5Ac started to be quickly synthesized when bacteria grown at 20 degrees C were transferred to 37 degrees C and was halted when cells grown at 37 degrees C were transferred to 20 degrees C. These findings suggest that temperature regulates the synthesis of this enzyme and therefore the concentration of CMP-Neu5Ac necessary for the biosynthesis of colominic acid.
大肠杆菌K - 235中结肠菌素酸的合成受到温度的严格调控。通过测量在20℃和37℃下生长的大肠杆菌中胞苷5'-单磷酸-N-乙酰神经氨酸（CMP-Neu5Ac）合成酶的水平，获得了该酶在这种调控中作用的证据。在20℃下生长的大肠杆菌中未发现活性。当在20℃下生长的细菌转移到37℃时，CMP-Neu5Ac开始快速合成，而当在37℃下生长的细胞转移到20℃时，合成停止。这些发现表明温度调节该酶的合成，从而调节结肠菌素酸生物合成所需的CMP-Neu5Ac的浓度。
