Bao Wenna, Pan Haifeng, Zhang Zhenhong, Cheng Yongqing, Xie Zhipeng, Zhang Jianguo
Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China; Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou 311106, Zhejiang, China.
Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
J Biosci Bioeng. 2015 May;119(5):538-42. doi: 10.1016/j.jbiosc.2014.10.013. Epub 2014 Nov 15.
A novel cis-epoxysuccinate hydrolase (CESH) producing strain of Labrys sp. BK-8 for production of L(+)-tartaric acid was isolated and identified. After optimization, a maximum activity of 3597 ± 151 U/g was achieved in batch culture in a 10 L fermentor. When Labrys sp. BK-8 was immobilized on κ-carrageenan, the immobilized cells showed a high conversion rate (>99%), enantioselectivity (EE > 99.5%) and storage stability (>90 d). A conversion rate of 97% was still achieved after 10 repeat batches. The CESH was stable over a broad range of temperatures (up to 45°C) and pH values (4.0-10.0). The Labrys sp. BK-8 isolate provides a new alternative with good stability for the industrial biosynthesis of L(+)-tartaric acid.
分离并鉴定了一株用于生产L(+)-酒石酸的新型产顺式环氧琥珀酸水解酶（CESH）的拉布立斯氏菌属菌株BK-8。经过优化，在10 L发酵罐的分批培养中，最大酶活达到3597±151 U/g。当将拉布立斯氏菌属菌株BK-8固定在κ-卡拉胶上时，固定化细胞表现出高转化率（>99%）、对映选择性（对映体过量值>99.5%）和储存稳定性（>90天）。经过10次重复批次后，仍实现了97%的转化率。CESH在较宽的温度范围（高达45°C）和pH值范围（4.0 - 10.0）内都很稳定。拉布立斯氏菌属菌株BK-8为L(+)-酒石酸的工业生物合成提供了一种具有良好稳定性的新选择。
