Tobia Chiara, Gariano Giuseppina, Guerra Jessica, Presta Marco
Department of Molecular and Translational Medicine, Experimental Oncology and Immunology, School of Medicine, University of Brescia, Brescia, Italy.
Methods Mol Biol. 2015;1214:173-84. doi: 10.1007/978-1-4939-1462-3_10.
Angiogenesis leads to the formation of the intersegmental vessels (ISVs) of the trunk in teleost zebrafish (Danio rerio) embryo. Here we describe experimental procedures, including in vivo observation of transgenic zebrafish embryo and whole mount in situ hybridization, to investigate ISV development in zebrafish embryos and assess the effect of antiangiogenic compounds on these vessels.
血管生成导致硬骨鱼斑马鱼（Danio rerio）胚胎躯干节间血管（ISV）的形成。在这里，我们描述了实验程序，包括对转基因斑马鱼胚胎的体内观察和全胚胎原位杂交，以研究斑马鱼胚胎中节间血管的发育，并评估抗血管生成化合物对这些血管的影响。
