Lee Jin Wuk, Kim Bo-Mi, Jeong Chang-Bum, Won Eun-Ji, Rhee Jae-Sung, Lee Jae-Seong
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea.
Comp Biochem Physiol C Toxicol Pharmacol. 2015 Jan;167:147-56. doi: 10.1016/j.cbpc.2014.10.005.
Acetlycholinesterase (AChE) is a serine esterase that plays an important role in the hydrolytic degradation of acetylcholine. We investigated the modulatory potential of T. japonicus-AChE (TJ-AChE) for biocide response by cloning, sequencing, and characterizing the full-length genomic DNA of the TJ-AChE1 and TJ-AChE2 genes. The deduced TJ-AChE proteins were highly conserved across species and were distinctively separated into two subtypes, AChE1 and AChE2. Each TJ-AChE protein was closely phylogenetically clustered with invertebrate AChE1 and AChE2 proteins. Transcriptional level of TJ-AChE1 was higher than TJ-AChE2 in all developmental stages. TJ-AChE1 mRNA decreased in response to five biocides (alachlor, chlorpyrifos, dimethoate, endosulfan, lindane,) but not in the molinate-exposed group. TJ-AChE2 decreased significantly only in response to chlorpyrifos and lindane. TJ-AChE enzymatic activity was significantly inhibited when exposed to alachlor, chlorpyrifos, endosulfan, or lindane for 24 h. This study elucidates potential endogenous mechanisms of biocide-induced neurotoxicity in T. japonicas.
乙酰胆碱酯酶（AChE）是一种丝氨酸酯酶，在乙酰胆碱的水解降解中起重要作用。我们通过克隆、测序和表征TJ-AChE1和TJ-AChE2基因的全长基因组DNA，研究了日本三角涡虫AChE（TJ-AChE）对杀生物剂反应的调节潜力。推导的TJ-AChE蛋白在物种间高度保守，并明显分为两种亚型，即AChE1和AChE2。每个TJ-AChE蛋白在系统发育上与无脊椎动物的AChE1和AChE2蛋白紧密聚类。在所有发育阶段，TJ-AChE1的转录水平均高于TJ-AChE2。TJ-AChE1 mRNA在暴露于五种杀生物剂（甲草胺、毒死蜱、乐果、硫丹、林丹）时下降，但在暴露于禾草敌的组中未下降。TJ-AChE2仅在暴露于毒死蜱和林丹时显著下降。当暴露于甲草胺、毒死蜱、硫丹或林丹24小时时，TJ-AChE酶活性受到显著抑制。本研究阐明了杀生物剂诱导日本三角涡虫神经毒性的潜在内源性机制。
