Colton C A, Fagni L, Gilbert D
Department of Physiology and Biophysics, Georgetown University Medical School, Washington, DC 20007.
Free Radic Biol Med. 1989;7(1):3-8. doi: 10.1016/0891-5849(89)90093-2.
The action of a reactive oxygen intermediate, that is, hydrogen peroxide (H2O2) on modulation of synaptic transmission was examined in the hippocampal brain slice preparation. Microinjection of H2O2 into the apical dendritic region of the CA1 pyramidal cells produced no change in either the pattern or amplitude of paired pulse facilitation compared to saline injection (control). Long term potentiation (LTP), induced by high frequency stimulation of homosynaptic inputs, however, was blocked by microinjection of H2O2 into the dendritic tree. LTP was seen in only 2 out of 10 slices investigated when treated with H2O2 while LTP was seen in 4 out of 5 slices when saline injected. The results suggest that a reactive oxygen intermediate can selectively modify synaptic mechanisms in the hippocampus.
在海马脑片标本中研究了活性氧中间体即过氧化氢（H2O2）对突触传递调节的作用。与注射生理盐水（对照）相比，向CA1锥体细胞的顶端树突区域微量注射H2O2，成对脉冲易化的模式或幅度均未发生变化。然而，通过高频刺激同突触输入诱导的长时程增强（LTP），会被向树突注入H2O2所阻断。在用H2O2处理的10个研究切片中，只有2个观察到LTP，而注射生理盐水时，5个切片中有4个观察到LTP。结果表明，活性氧中间体能选择性地改变海马体中的突触机制。
