匈牙利南部自然啮齿动物和蜱虫群落中的嗜吞噬细胞无形体及新埃立克体候选种。
Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary.
作者信息
Szekeres Sándor, Claudia Coipan Elena, Rigó Krisztina, Majoros Gábor, Jahfari Setareh, Sprong Hein, Földvári Gábor
机构信息
Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, 2 István Street H-1078, Budapest, Hungary.
Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and Environment, 9 Antonie van Leeuwenhoeklaan, P.O. Box 1, Bilthoven, The Netherlands.
出版信息
Ticks Tick Borne Dis. 2015 Mar;6(2):111-6. doi: 10.1016/j.ttbdis.2014.10.004. Epub 2014 Nov 14.
The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p<0.05). Among questing Ixodes ricinus ticks we found three (8.8%) individuals (female, male, nymph) infected with Candidatus N. mikurensis. Five (3.1%) questing ticks had A. phagocytophilum infection (one I. ricinus male, two Dermacentor reticulatus females and two Haemaphysalis concinna females). We found one I. ricinus nymph removed from a male A. flavicollis with A. phagocytophilum infection. Our study provides new data on the occurrence of these pathogens in rodent tissue samples, questing ticks and engorged ticks in Southern Hungary.
本研究的目的是调查匈牙利南部新出现的人类致病细菌米库新埃立克体（Candidatus Neoehrlichia mikurensis）和嗜吞噬细胞无形体（Anaplasma phagocytophilum）的自然循环。我们在2010年至2013年期间用活饵诱捕器捕获啮齿动物，并于2012年用拖旗法采集伺机侵袭的蜱。对小型哺乳动物实施安乐死后，采集组织样本，并清除所有体表寄生虫，保存在70%乙醇中。我们发现蜱侵袭的总体发生率相对较低（8%）。分别针对主要表面蛋白2（msp2）的一部分和热休克蛋白groEL基因，采用多重定量实时PCR对样本进行嗜吞噬细胞无形体和米库新埃立克体分析。嗜吞噬细胞无形体在组织样本中的总体发生率为皮肤6.6%、脾脏5.1%，米库新埃立克体为皮肤1.7%、脾脏3.4%。仅在黄颈姬鼠（Apodemus flavicollis）和黑线姬鼠（Apodemus agrarius）中检测到米库新埃立克体，而在黄颈姬鼠、黑线姬鼠、林姬鼠（Myodes glareolus）、普通田鼠（Microtus arvalis）和小家鼠（Mus musculus）样本中发现了嗜吞噬细胞无形体。黄颈姬鼠皮肤样本中嗜吞噬细胞无形体的发生率显著高于米库新埃立克体（p<0.05）。在伺机侵袭的蓖麻硬蜱（Ixodes ricinus）中，我们发现3只（8.8%）个体（雌蜱、雄蜱、若蜱）感染了米库新埃立克体。5只（3.1%）伺机侵袭的蜱感染了嗜吞噬细胞无形体（1只蓖麻硬蜱雄蜱、2只网纹革蜱（Dermacentor reticulatus）雌蜱和2只全沟硬蜱（Haemaphysalis concinna）雌蜱）。我们发现1只从感染嗜吞噬细胞无形体的雄性黄颈姬鼠身上采集的蓖麻硬蜱若蜱。我们的研究提供了关于这些病原体在匈牙利南部啮齿动物组织样本、伺机侵袭的蜱和饱血蜱中发生情况的新数据。
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