Rohozinski J, Van Etten J L
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Intervirology. 1989;30(3):156-62. doi: 10.1159/000150087.
Chlorella NC64A cells infected with the large double-stranded DNA-containing virus PBCV-1 and uninfected cells were assayed for DNA polymerase activity. Both uninfected and infected cells contained three forms of DNA polymerase activity: (i) an exogenous DNA-dependent 10,000 g soluble fraction, (ii) an exogenous DNA-dependent 10,000 g particulate fraction, and (iii) a DNA-independent 10,000 g particulate fraction. The three DNA polymerase activities in the infected and uninfected cells were distinguished from one another by the conditions required for optimum activity and by their sensitivity to inhibitors.
对感染了含双链DNA的大型病毒PBCV - 1的小球藻NC64A细胞和未感染的细胞进行了DNA聚合酶活性检测。未感染和感染的细胞均含有三种形式的DNA聚合酶活性：（i）一种外源DNA依赖性的10,000g可溶性组分，（ii）一种外源DNA依赖性的10,000g颗粒性组分，以及（iii）一种不依赖DNA的10,000g颗粒性组分。感染和未感染细胞中的这三种DNA聚合酶活性可通过最佳活性所需条件及其对抑制剂的敏感性来相互区分。
