病毒PBCV-1的小球藻受体特性

Properties of the Chlorella receptor for the virus PBCV-1.

作者信息

Meints R H, Burbank D E, Van Etten J L, Lamport D T

机构信息

School of Biological Sciences, Department of Plant Pathology, University of Nebraska, Lincoln 68588-0118.

出版信息

Virology. 1988 May;164(1):15-21. doi: 10.1016/0042-6822(88)90614-9.

DOI:10.1016/0042-6822(88)90614-9

PMID:3363863

Abstract

The virus PBCV-1 attached rapidly, specifically, and irreversibly to the external surface of cell walls of its host, a unicellular, eukaryotic Chlorella-like green alga. Attachment was pH and salt dependent. Each cell contained at least 5 X 10(4) PBCV-1 binding sites and Scatchard analysis indicated that each cell could adsorb 5000 PBCV-1 particles. The PBCV-1 receptor was unaffected by extraction with organic solvents, detergents, high salts, or treatment with several proteases as well as the polysaccharide degrading enzymes, cellulase and pectinase. In contrast, acid and alkali treatments of walls at high temperatures and treatment with an enzyme preparation from PBCV-1 lysates destroyed the virus receptor. We suspect that the receptor is a carbohydrate.

摘要

病毒PBCV-1能迅速、特异性且不可逆地附着于其宿主（一种单细胞真核绿藻，类似小球藻）细胞壁的外表面。附着过程依赖于pH值和盐浓度。每个细胞至少含有5×10⁴个PBCV-1结合位点，斯卡查德分析表明每个细胞可以吸附5000个PBCV-1颗粒。PBCV-1受体不受有机溶剂萃取、去污剂、高盐处理的影响，也不受几种蛋白酶以及多糖降解酶（纤维素酶和果胶酶）处理的影响。相比之下，高温下细胞壁的酸碱处理以及用PBCV-1裂解物中的酶制剂处理会破坏病毒受体。我们怀疑该受体是一种碳水化合物。