Mehraban Fouad, Jafari Mehrzad, Akbartabar Toori Mehdi, Sadeghi Hossein, Joodi Behzad, Mostafazade Mostafa, Sadeghi Heibatollah
Medicinal Plant Research Center, Yasuj University of Medical Sciences, Yasuj, Iran ; Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.
Iran J Reprod Med. 2014 Oct;12(10):705-12.
Date Palm Pollen (DPP) and Astragalus genus are used in some countries for the treatment of infertility.
This study was designed to investigate effects of DPP and Astragalus ovinus (A.Ovinus) on fertility in healthy adult male rats.
Thirty-six rats were divided into six groups (n=6) including control and five treatment groups. DPP (120, 240 and 360 mg/kg) and A.ovinus (100, 500 mg/ kg) were orally given to the treatment groups. After thirty-five days, blood samples were taken to determine serum levels of FSH, LH, testosterone and estradiol. Weight of testis and epididymis, sperm count, sperm motility, seminiferous tubules diameter (STD), germinal cell layer thickness (GCLT), sertoli, leydig and spermatogonia cells were also evaluated.
DPP at the of 120 and 240 mg/kg doses significantly raised the ratio of testis or epididymis to body weight, sperm count, sperm motility , and estradiol level compared to the control group (p<0.05). LH and testosterone levels only noticeably increased at 120 mg/kg of DPP (p<0.01 and p<0.001 respectively). STD increased in the three applied doses (p=0.001). A. ovinus extract at the indicated doses produced a significant reduction in the ratio of testis or epididymis to body weight and sperm motility (p<0.05). Sperm count, spermatogonia, leydig cells and FSH level decreased at dose of 500 mg/kg. Furthermore, GCLT, spermatogonia cells, and serum estradiol level increased at 100 mg/kg dose of A. ovinus.
Our findings indicate that DPP could improve fertility factors, while A.ovinus can exhibit deleterious effects on gonad and sperm parameters in rats.
在一些国家，海枣花粉（DPP）和黄芪属植物被用于治疗不孕症。
本研究旨在调查DPP和绵毛黄芪（A. Ovinus）对健康成年雄性大鼠生育能力的影响。
将36只大鼠分为六组（n = 6），包括对照组和五个治疗组。给治疗组口服DPP（120、240和360 mg/kg）和A. ovinus（100、500 mg/kg）。35天后，采集血样以测定血清促卵泡生成素（FSH）、促黄体生成素（LH）、睾酮和雌二醇水平。还评估了睾丸和附睾的重量、精子计数、精子活力、曲细精管直径（STD）、生殖细胞层厚度（GCLT）、支持细胞、间质细胞和精原细胞。
与对照组相比，120和240 mg/kg剂量的DPP显著提高了睾丸或附睾与体重的比值、精子计数、精子活力和雌二醇水平（p < 0.05）。仅在120 mg/kg的DPP剂量下，LH和睾酮水平显著升高（分别为p < 0.01和p < 0.001）。三个应用剂量下STD均增加（p = 0.001）。所示剂量的A. ovinus提取物使睾丸或附睾与体重的比值和精子活力显著降低（p < 0.05）。在500 mg/kg剂量下，精子计数、精原细胞、间质细胞和FSH水平降低。此外，在100 mg/kg剂量的A. ovinus下，GCLT、精原细胞和血清雌二醇水平增加。
我们的研究结果表明，DPP可以改善生育因素，而A. ovinus对大鼠的性腺和精子参数可能具有有害影响。
