Liu Chang, Li Nan, Lu Heng, Wang Zhengkai, Chen Chunyan, Wu Lin, Liu Jiong, Lu Youke, Wang Fangyu
Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
Biomed Rep. 2015 Jan;3(1):123-127. doi: 10.3892/br.2014.388. Epub 2014 Nov 17.
The secreted frizzled-related protein 1 () gene plays an important role in carcinogenesis of digestive system cancer. Previous studies proved that circulating DNA promoter methylation may be a suitable biomarker for cancer patients. The aim of the present study was to investigate whether the promoter methylation status of serum is a potential biomarker for gastric adenocarcinoma (GAC) and esophageal square cell carcinoma (ESCC). The blood samples obtained from 42 GAC and 36 ESCC patients were detected for the promoter methylation status of by methylation-specific polymerase chain reaction. The control group included 42 benign gastrointestinal disease volunteers (24 benign gastric disease and 18 benign esophageal disease) and 20 healthy volunteers. Serum methylation was evident in 30.95% (13/42) GAC patients and 38.89% (14/36) ESCC patients, which is clearly higher compared to 8.33% (2/24) in benign gastric disease, 11.11% (2/18) in benign esophageal disease and 5% (1/20) in healthy volunteers (P<0.05). The association between the serum promoter methylation status and the clinical pathological features were further analyzed and methylation of the gene was significantly associated with age >60 years in GAC patients (P=0.027). However, no correlations between the methylation status and other clinicopathological parameters were found. In conclusion, the promoter was detected frequently in the serum of GAC and ESCC patients. The detection of circulating methylated in the serum may be a useful biomarker for upper gastrointestinal cancer patients.
分泌型卷曲相关蛋白1（）基因在消化系统癌症的发生发展中起重要作用。先前的研究证明，循环DNA启动子甲基化可能是癌症患者合适的生物标志物。本研究旨在探讨血清启动子甲基化状态是否是胃腺癌（GAC）和食管鳞状细胞癌（ESCC）的潜在生物标志物。通过甲基化特异性聚合酶链反应检测42例GAC患者和36例ESCC患者的血液样本中启动子甲基化状态。对照组包括42名良性胃肠道疾病志愿者（24例良性胃病和18例良性食管病）和20名健康志愿者。30.95%（13/42）的GAC患者和38.89%（14/36）的ESCC患者血清甲基化明显，明显高于良性胃病患者的8.33%（2/24）、良性食管病患者的11.11%（2/18）和健康志愿者中的5%（1/20）（P<0.05）。进一步分析血清启动子甲基化状态与临床病理特征之间的关联，发现GAC患者中基因甲基化与年龄>60岁显著相关（P=0.027）。然而，未发现甲基化状态与其他临床病理参数之间的相关性。总之，在GAC和ESCC患者血清中频繁检测到启动子。检测血清中循环甲基化可能是上消化道癌症患者有用的生物标志物。
