Chang T C
Taiwan Yi Xue Hui Za Zhi. 1989 Jan;88(1):13-7.
Thyroid tissues excised from 5 patients with Graves' disease were used to study the influence of lithium carbonate on the thyrotropin (TSH) receptor in vitro by cAMP generation assay and TSH binding to its receptor. Lithium carbonate was able to decrease the cAMP production of the crude thyroid plasma membranes stimulated by TSH at concentrations of 6mM and higher. However, lithium carbonate had no effect on 5'-guanylylimidodiphosphate-induced cAMP production in thyroid plasma membranes up to concentrations of 10mM. Lithium carbonate was able to decrease the binding of 125I-TSH to crude thyroid plasma membranes at concentrations of 6mM and higher. It decreased the binding of 125I-TSH to solubilized TSH receptor at concentrations of 6mM and higher. These findings suggest that lithium carbonate can influence the TSH receptor in vitro and may be one of the action mechanisms of lithium on the thyroid gland.
从5例格雷夫斯病患者切除的甲状腺组织用于通过环磷酸腺苷（cAMP）生成测定和促甲状腺激素（TSH）与其受体的结合来体外研究碳酸锂对TSH受体的影响。在6mM及更高浓度下，碳酸锂能够降低TSH刺激的粗甲状腺质膜的cAMP产生。然而，在浓度高达10mM时，碳酸锂对甲状腺质膜中5'-鸟苷酰亚胺二磷酸诱导的cAMP产生没有影响。在6mM及更高浓度下，碳酸锂能够降低125I-TSH与粗甲状腺质膜的结合。在6mM及更高浓度下，它降低了125I-TSH与可溶性TSH受体的结合。这些发现表明碳酸锂在体外可影响TSH受体，可能是锂对甲状腺作用的机制之一。
